A fertilization method for improving secondary salinized greenhouse soil
A soil and organic fertilizer technology, which is applied in the field of fertilization to improve secondary salinized greenhouse soil, and can solve problems such as decline, crop production reduction, and agricultural product quality
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[0049] Embodiment 1, improving secondary salinization greenhouse soil (alkaline greenhouse soil)
[0050] 1. Preparation of Paenibacillus jamiri inoculum
[0051] The preparation method of the culture medium used in this embodiment is as follows:
[0052] Beef extract medium: glucose 20g, peptone 15g, sodium chloride 5g, beef extract 0.5g, agar 20g, distilled water to 1L, pH 7.0, sterilized at 121°C for 15min.
[0053] Fermentation medium of Paenibacillus: sucrose 15g, soybean meal 10g, (NH 4 ) 2 SO 4 4g,K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.1g, distilled water to 1L, pH 6.5, sterilized at 121°C for 15min.
[0054] Inoculate Paenibacillus jamilae (Paenibacillus jamilae) ACCC 03134 on a beef extract culture plate, culture at 37°C for 72 hours, then scrape off the spores on a culture plate and insert it into 500 mL of fermentation medium containing 200 mL of Paenibacillus jamilae In a triangular flask, cultivate for 72h at 37°C and 220rpm; then inoculate 2% (v / v) inoculum ...
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