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Construction method of double-disease model mouse

A technology of disease model and construction method, applied in the field of construction of double-disease model mice, can solve the problems of prone to complications, high operator requirements, associated with Parkinson's disease, etc., and achieves long stabilization time and high success rate. , the effect of easy detection

Active Publication Date: 2021-04-02
FUJIAN ACAD OF MEDICAL SCI
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Problems solved by technology

Surgical modeling has high requirements for operators and is prone to complications. The success rate of virus-induced modeling is not high, there are side effects, and the cycle of fattening and diet induction is long
Type 1 diabetes patients will be accompanied by Parkinson's disease, and the mouse model of type 1 diabetes combined with Parkinson's disease has not been reported yet

Method used

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  • Construction method of double-disease model mouse
  • Construction method of double-disease model mouse
  • Construction method of double-disease model mouse

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Experimental program
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Effect test

Embodiment 1

[0027] 1. NOD / LtJ Mice mice were purchased from Wu's Experimental Animals (http: / / www.wssydw.com), 3 weeks old female mice were randomly divided into 3 groups: blank control group, MFB mechanical injury group (positive control group) ), drinking water group (experimental group), 12 rats in each group, adaptive feeding for one week, and the formal experiment started on day 28. Dimethyl itaconate was purchased from Shanghai Yien Chemical Technology Co., Ltd. (product number: R000622-25g, content: 98%).

[0028] 2. Preparation method of MFB mechanical injury model group: After being accurately fixed on the brain stereotaxic instrument, use a wire knife to go deep into the MFB site to cut it off; Drinking water group: 2 days / change water (containing 1.5mM itaconic acid di Methyl ester), the time is 15-20 days, individual differences are slightly different in different times; during the period of spontaneous type 1 diabetes, strengthen drinking water (once a week, for 2 days, until...

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Abstract

The invention provides a construction method of a double-disease model mouse. Dimethyl itaconate is dissolved in double distilled water, the concentration is 1.5 mM, the mouse is fed, new water is replaced every two days, and the feeding time is 15-20 days; and during the period of spontaneous type 1 diabetes mellitus, intensive water drinking is carried out once a week, the time is 2 days, and the double-disease model mouse is finally obtained. By means of the model, the injury of a motion function of the model can be easily detected, the injury includes motion retardation, muscular stiffness, static tremor and other main symptoms of PD, the success rate is high, and the model stabilization time is long.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing dual-disease model mice. Background technique [0002] Diabetes has seriously threatened human life and health, and is the most serious chronic non-communicable disease after tumors and cardiovascular diseases. With the development of my country's social economy, the improvement of people's living standards and the change of diet structure, the incidence of diabetes in my country is as high as 9.7%. According to a survey in 2010, China currently has more than 92.4 million diabetic patients and has become the world's largest diabetic country. Diabetes animal models help to further reveal the pathogenesis and intervention measures of diabetes, and test and screen various hypoglycemic drugs. Animal models can be divided into spontaneous and experimental. Compared with experimental models, spontaneous models are less popular and cost more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2227/105A01K2207/20A01K2267/0362A01K2267/0356Y02A50/30
Inventor 韩俊永陈金烟王坤薛士杰金静君
Owner FUJIAN ACAD OF MEDICAL SCI
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