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A culture medium and culture method for inducing umbilical cord mesenchymal stem cell factor secretion

A technology of mesenchymal stem cells and culture methods, which is applied in the field of medium for inducing umbilical cord mesenchymal stem cell factor secretion, can solve the problems of time-consuming and labor-intensive, increase safety hazards of cytokine products, and achieve the effects of promoting secretion, easy operation, and increasing content

Active Publication Date: 2022-04-22
新疆赛尔托马斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bovine serum, human serum or other animal serum may contain blood-borne pathogens, and bovine serum can also stimulate the production of antibodies against xenobiotic proteins, which can stimulate immune responses in recipient patients, increasing the safety of cytokine products hidden danger, and because of the low content of cytokines in the culture medium, a large amount of cell culture is often required to achieve the corresponding effect, which is time-consuming and laborious, so it is necessary to improve the medium to induce the secretion of umbilical cord mesenchymal stem cells More cytokines, lower production costs, and improved safety of cytokine products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of culture medium that induces the secretion of umbilical cord mesenchymal stem cell factors, by basal medium DMEM / F12 (the volume ratio of DMEM and F12 is 1:1), add gossypol acetate, meglumine, dexamethasone in the basal medium Glycoside and dipotassium glycyrrhizinate, based on the final concentration of the medium, the dosage of each component is: gossypol acetate 47.5μg / mL, meglumine 84.5ng / mL, dextran 25.8μg / mL, glycyrrhizinic acid di Potassium 3mg / mL.

[0029] A culture method for inducing the secretion of umbilical cord mesenchymal stem cells, comprising the following steps: after the umbilical cord mesenchymal stem cells subcultured to the P3 generation are washed with PBS, the cells are inoculated into the above-mentioned induced umbilical cord mesenchymal stem cells in T75 culture flasks. In the medium secreted by stem cell factor, the seeding density of cells was 1.5×10 5 cells / mL, at 37°C, 5% CO 2 Induction culture was carried out in the incubator f...

Embodiment 2

[0031] A kind of culture medium that induces the secretion of umbilical cord mesenchymal stem cell factors, by basal medium DMEM / F12 (the volume ratio of DMEM and F12 is 1:1), add gossypol acetate, meglumine, dexamethasone in the basal medium Glycoside and dipotassium glycyrrhizinate, based on the final concentration of the medium, the dosage of each component is: gossypol acetate 45.3μg / mL, meglumine 78.5ng / mL, dextran 23.8μg / mL, glycyrrhizinic acid di Potassium 1mg / mL.

[0032] A culture method for inducing the secretion of umbilical cord mesenchymal stem cells, comprising the following steps: after the umbilical cord mesenchymal stem cells subcultured to the P4 generation are washed with PBS, the cells are inoculated into the above-mentioned induced umbilical cord mesenchymal stem cells in T75 culture flasks. In the medium secreted by stem cell factor, the seeding density of cells was 1.0×10 5 cells / mL, at 37°C, 5% CO 2 Induction culture was carried out in the incubator f...

Embodiment 3

[0034] A kind of culture medium that induces the secretion of umbilical cord mesenchymal stem cell factors, by basal medium DMEM / F12 (the volume ratio of DMEM and F12 is 1:1), add gossypol acetate, meglumine, dexamethasone in the basal medium Glycoside and dipotassium glycyrrhizinate, based on the final concentration of the medium, the dosage of each component is: gossypol acetate 50.5μg / mL, meglumine 90.0ng / mL, dextran 28.2μg / mL, glycyrrhizic acid di Potassium 5mg / mL.

[0035] A culture method for inducing the secretion of umbilical cord mesenchymal stem cells, comprising the following steps: after the umbilical cord mesenchymal stem cells subcultured to the P5 generation are washed with PBS, the cells are inoculated into the above-mentioned induced umbilical cord mesenchymal stem cells in T75 culture flasks. In the medium secreted by stem cell factor, the seeding density of cells was 2×10 5 cells / mL, at 37°C, 5% CO 2 Induction culture was carried out in the incubator for 3...

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Abstract

The invention discloses a medium for inducing factor secretion of umbilical cord mesenchymal stem cells, which comprises a base medium and gossypol acetate, meglumine, dextran, and dipotassium glycyrrhizinate added to the base medium. The present invention provides a medium for inducing the secretion of umbilical cord mesenchymal stem cell factors, in which gossypol acetate, meglumine, dextran, and dipotassium glycyrrhizinate are added to the basal medium. The induced culture process of mesenchymal stem cells can effectively promote the secretion of cytokines, increase the content of cytokines in cell culture medium, and provide guarantee for the application of umbilical cord mesenchymal stem cell factor products in the field of beauty and skin care. The present invention also provides a culture method for inducing factor secretion of umbilical cord mesenchymal stem cells. Using the induction medium of the present invention, the subcultured umbilical cord mesenchymal stem cells are used as the induction object. The whole process is easy to operate, saves cost and is convenient for business. Chemical production of various cytokine products.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a culture medium and a culture method for inducing the secretion of factors of umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells were first discovered in the bone marrow, and have attracted increasing attention due to their multiple differentiation potentials, hematopoietic support, promotion of stem cell implantation, immune regulation, and self-replication. Bone marrow mesenchymal stem cells have been widely used clinically, and current research shows that umbilical cord-derived mesenchymal stem cells will also have greater application potential. [0003] Human umbilical cord mesenchymal stem cells express a variety of unique markers of stem cells. They have the characteristics of high differentiatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12P21/02C07K14/485C07K14/50C07K14/475C07K14/495
CPCC12N5/0665C12P21/02C07K14/485C07K14/50C07K14/475C07K14/495C12N2501/999
Inventor 夏爽梁杰
Owner 新疆赛尔托马斯生物科技有限公司
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