Unlock instant, AI-driven research and patent intelligence for your innovation.

A Oil Hydrocarbon Degrading Functional Bacteria scsio 19801 and Its Application

A technology of SCSIO19801, cgmccno.21142, applied in the direction of bacteria, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problem of low degradation ability of petroleum hydrocarbons

Active Publication Date: 2022-04-15
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported Gordonella mainly comes from land and has low ability to degrade petroleum hydrocarbons. Therefore, the development of marine-sourced strains that can efficiently degrade petroleum hydrocarbons is of great significance for the in-situ remediation of oil spill pollution.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Oil Hydrocarbon Degrading Functional Bacteria scsio 19801 and Its Application
  • A Oil Hydrocarbon Degrading Functional Bacteria scsio 19801 and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Screening and Identification of Gordonia sp. SCSIO 19801 Strains

[0020] Preparation of inorganic salt medium MSM (g / L): K 2 HPO 4 , 0.27; KH 2 PO 4 , 0.35; NH 4 Cl, 2.7; MgCl 2 ·6H 2 O, 0.1; CaCl 2 2H 2 O, 0.1; FeCl 2 4H 2 O, 0.009; MnCl 2 4H 2 O, 0.004; ZnCl 2 , 0.0014; CoCl 4 ·6H 2 O, 0.001; (NH 4 ) 6 Mo 7 o 24 4H 2 O, 0.001; pH 7.0. Add 1% (V:V) petroleum and diesel oil to the inorganic salt medium to prepare enriched medium. The seawater samples collected on the surface of islands and reefs in the South China Sea were added to the enrichment medium, and the enrichment culture was cultured at 28°C for 15 days. The enriched culture was transferred to the above-mentioned fresh enriched medium with an inoculum volume of 1% by volume, cultured under the same conditions, and the enriched culture was repeated for 3 times. The culture after the third transfer culture was applied to LB medium, and after 2-3 days of culture, colonies with di...

Embodiment 2

[0025] Embodiment 2: Determination of the ability of Gordonia sp. SCSIO 19801 strain to degrade alkanes and polycyclic aromatic hydrocarbons

[0026] Prepare inorganic salt medium MSM and sterilize it at high temperature, add polycyclic aromatic hydrocarbons (phenanthrene, pyrene), 1% (V:V) diesel oil and liquid alkanes (n-dodecane, n-hexadecane) with a final concentration of 100 mg / L respectively ) to obtain the test medium. Add 1% by volume OD 600 =0.6 Gordonia sp. SCSIO19801 bacterium solution was inserted into the above test medium, cultured at 28° C. for 7 days, and residual petroleum hydrocarbons in the culture were detected by methods such as GC-MS. The results showed that the degradation rate of Gordonia sp. SCSIO 19801 strain to phenanthrene was 83%, the degradation rate to pyrene was 51%, the degradation rate to n-dodecane was 26%, and the degradation rate to n-hexadecane The degradation rate of diesel oil is 12%, and the degradation rate of diesel oil is 23%, see ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a petroleum hydrocarbon degrading functional bacterium SCSIO 19801 and its application. The Gordonia sp. SCSIO 19801 of the present invention has the highest similarity (100%) to Gordonia terrae, and the strain was deposited in the General Microorganism Center of the China Committee for Microbial Culture Collection on December 07, 2020, and the preservation number is : CGMCC No.21142. The strain (Gordonia sp.) SCSIO 19801 has the ability to efficiently degrade petroleum hydrocarbons, and can be widely used in the degradation of petroleum hydrocarbons and the restoration of oil pollution, such as the restoration of oil-polluted water bodies in marine environments.

Description

Technical field: [0001] The invention belongs to the field of biotechnology and relates to biological treatment of petroleum pollution, in particular to a strain of Gordonia sp. SCSIO 19801 for degrading petroleum hydrocarbons and its application. Background technique: [0002] According to statistics, the world's annual oil entering the ocean is 1.0×10 10 More than kg, marine oil spill has become a serious worldwide marine pollution. The Ministry of Ecology and Environment's bulletin on the status of China's marine ecological environment in recent years shows that petroleum has become one of the main pollutants in my country's oceans. Marine transportation (ship oil spills, ship and wharf operation sewage discharge, etc.), offshore oil exploitation, and land-based oil pollution input are the main sources of marine oil spill pollution. A large area of ​​oil spill spreads on the sea surface to form an oil film, which weakens the energy of solar radiation penetrating into se...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32C02F103/08
CPCC02F3/344C02F2103/08C02F2101/327C02F2101/32
Inventor 张偲麦志茂吕丽娜王琳李洁
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI