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Method for preparing chondroitin sulfate from squid cartilage

A technology for chondroitin sulfate and squid cartilage, which is applied in the field of preparing chondroitin sulfate by using squid cartilage, can solve the problems of complex extraction process, low product yield and high protein content, and achieves simple process flow, high product purity and high purity. Effect

Inactive Publication Date: 2021-04-09
荣成万盈水产科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of complex extraction process, low product yield and higher protein content than 5% in the traditional method of extracting chondroitin sulfate, the purpose of the present invention is to provide a method for preparing chondroitin sulfate using squid cartilage

Method used

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  • Method for preparing chondroitin sulfate from squid cartilage
  • Method for preparing chondroitin sulfate from squid cartilage
  • Method for preparing chondroitin sulfate from squid cartilage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of method utilizing squid cartilage to prepare chondroitin sulfate, it goes through the following processing steps:

[0035] 1) Preparation of chondroitin sulfate lyase

[0036] a. Take fresh duck small intestines, taken from ducks that have been fed with shrimp and crab shells for 2 years, crush them with a wall breaker, add sterile water in parts by weight 1:5.5 to prepare duck small intestine solution, stir for 15 minutes, and use 180 mesh Filter with a sieve, then take the filtrate to coat the medium, carry out plate culture and isolation, then pick a single colony, and purify it by streaking on a slant; prepare a medium containing squid cartilage powder, and plant the purified single colony on the medium plate , 32°C constant temperature culture for 2 days, pour sterile water into the plate, soak for 4 minutes; pick the strains that produce transparent circles, use the inclined plane to culture, control the temperature at 32°C, 130r / min shake aerobic culture...

Embodiment 2

[0048] A kind of method utilizing squid cartilage to prepare chondroitin sulfate, it goes through the following processing steps:

[0049] 1) Preparation of chondroitin sulfate lyase

[0050] a. Take fresh duck small intestines, taken from ducks that have been fed with shrimp and crab shells for 2 years, crush them with a wall breaker, add sterile water at a ratio of 1:5 by weight to prepare duck small intestine solution, stir for 10 minutes, and use 160 mesh Filter with a sieve, then take the filtrate to coat the medium, carry out plate culture and isolation, then pick a single colony, and purify it by streaking on a slant; prepare a medium containing squid cartilage powder, and plant the purified single colony on the medium plate , 30°C constant temperature culture for 1 day, pour sterile water into the plate, soak for 3 minutes; pick the strains that produce transparent circles, use the inclined plane to culture, control the temperature at 30°C, 120r / min shaking aerobic cul...

Embodiment 3

[0062] A kind of method utilizing squid cartilage to prepare chondroitin sulfate, it goes through the following processing steps:

[0063] 1) Preparation of chondroitin sulfate lyase

[0064] a. Take fresh duck small intestines, taken from ducks that have been fed with shrimp and crab shells for 3 years, crush them with a wall breaker, add sterile water in parts by weight 1:6 to prepare duck small intestine solution, stir for 20 minutes, and use 180 mesh Filter with a sieve, then take the filtrate to coat the medium, carry out plate culture and isolation, then pick a single colony, and purify it by streaking on a slant; prepare a medium containing squid cartilage powder, and plant the purified single colony on the medium plate , 37 ℃ constant temperature culture for 3 days, pour sterile water into the plate, soak for 5 minutes; pick the strains that produce transparent circles, use the slope to culture, control the temperature at 37 ℃, 150r / min shake aerobic culture for 3 days...

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Abstract

The invention discloses a method for preparing chondroitin sulfate from squid cartilage, which solves the problems of complex extraction process, low product yield and protein content higher than 5% in the traditional method for extracting chondroitin sulfate. The method comprises the following process steps: 1) preparing chondroitin sulfate lyase; 2) carrying out enzymolysis on the squid cartilage; 3) purifying; and 4) drying. Squid cartilage is used as a raw material, the chondroitin sulfate lyase is extracted from small intestines of ducks and applied to enzymolysis of the squid cartilage to prepare chondroitin sulfate, and a fermentation product is purified for the first time through alkoxide, purified for the second time through a combined membrane electric field and dried to obtain the product. A biological method and a combined purification method are adopted, the extraction rate is high, the biological activity is high, and the prepared chondroitin sulfate is mainly chondroitin sulfate C. The method is simple and efficient in technological process, easy to control and beneficial to large-scale popularization and production.

Description

technical field [0001] The invention relates to the preparation of chondroitin sulfate, in particular to a method for preparing chondroitin sulfate from squid cartilage. Background technique [0002] Squid processing is often accompanied by a large number of by-products, including cartilage, inner shell, etc. These wastes are rarely used, except for some processed into fish meal or feed, because they are perishable and deteriorated, they are treated as garbage, resulting in a waste of resources . Studies have shown that the main components of squid cartilage are protein and chondroitin sulfate, of which chondroitin is a biomedical product that has various activities such as lowering blood fat and preventing vascular sclerosis. Therefore, strengthening comprehensive utilization and turning waste into treasure can effectively increase its added value, reduce the cost of waste treatment, and promote the development of squid processing. Because squid cartilage has a wide sourc...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0069C08B37/0003
Inventor 吴文惠杨劲峰
Owner 荣成万盈水产科技有限公司
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