Preparation method of squid chondroitin sulfate glycoprotein

A technology of chondroitin sulfate and squid cartilage, which is applied in the field of extracting glycoproteins, can solve the problems of low extraction rate, high protein content, complicated process, etc., and achieve the effects of high extraction rate, high product purity and simple process flow

Pending Publication Date: 2021-03-12
荣成万盈水产科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the shortcomings of complex process, low extraction rate and high protein content in the traditional method for extracting chondroitin sulfate glycoprotein, the purpose of the present invention is to provide a method for preparing squid chondroitin sulfate glycoprotein. The present invention adopts biological method And combined purification method, high extraction rate, strong biological activity, the prepared chondroitin sulfate is mainly chondroitin sulfate C, the process is simple and efficient, easy to control, and conducive to large-scale production

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  • Preparation method of squid chondroitin sulfate glycoprotein
  • Preparation method of squid chondroitin sulfate glycoprotein
  • Preparation method of squid chondroitin sulfate glycoprotein

Examples

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Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of squid chondroitin sulfate glycoprotein, which goes through the following processing steps:

[0031] 1) Preparation of chondroitin sulfate lyase-producing bacteria

[0032] Take fresh duck small intestines, taken from ducks fed with shrimp and crab shells for 2 years, crush them with a wall breaker, add sterile water at 1:5.5 by weight to prepare duck small intestine solution, stir for 18 minutes, and filter through a 180-mesh sieve , then take the filtrate coating culture medium, carry out plate culture separation, then pick a single colony, and purify with the method of slant streaking; prepare a medium containing squid cartilage powder, plant the purified single colony on the medium plate, 32 Cultivate at a constant temperature of ℃ for 1 day, pour sterile water into the plate, and soak for 4 minutes; pick out the strains that produce transparent circles, use a slant to culture, control the temperature at 32 ℃, 140r / min shake aerobic culture fo...

Embodiment 2

[0043] A preparation method of squid chondroitin sulfate glycoprotein is characterized in that it undergoes the following processing steps:

[0044] 1) Preparation of chondroitin sulfate lyase-producing bacteria

[0045]Take fresh duck small intestines, taken from ducks that have been fed with shrimp and crab shells for 2 years, crush them with a wall breaker, add sterile water at a ratio of 1:5 by weight to prepare duck small intestine solution, stir for 10 minutes, and filter through a 160-mesh sieve , then take the filtrate coating medium, carry out plate culture separation, then pick a single colony, and purify it with the slant method; prepare a medium containing squid cartilage powder, plant the purified single colony on the medium plate, 30 Cultivate at a constant temperature of ℃ for 1 day, pour sterile water into the plate, and soak for 3 minutes; pick the strain that produces a transparent circle, use a slant to culture, control the temperature at 30 ℃, 120r / min shak...

Embodiment 3

[0056] A preparation method of squid chondroitin sulfate glycoprotein is characterized in that it undergoes the following processing steps:

[0057] 1) Preparation of chondroitin sulfate lyase-producing bacteria

[0058] Take fresh duck small intestines, taken from ducks that have been fed with shrimp and crab shells for 3 years, crush them with a wall breaker, add sterile water at a ratio of 1:6 by weight to prepare duck small intestine solution, stir for 20 minutes, and filter through a 180-mesh sieve , then take the filtrate to coat the medium, carry out plate culture and isolation, then pick a single colony, and purify it with the method of streaking on a slant; prepare a medium containing squid cartilage powder, plant the purified single colony on the medium plate, 37 Cultivate at a constant temperature of ℃ for 3 days, pour sterile water into the plate, and soak for 5 minutes; pick out the strains that produce transparent circles, use a slant to culture, control the temp...

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Abstract

The invention discloses a preparation method of squid chondroitin sulfate glycoprotein, which solves the problems of complex process, low extraction rate and high protein content in the traditional method for extracting chondroitin sulfate glycoprotein. The method comprises the following process steps: 1) preparing chondroitin sulfate lyase producing bacteria; 2) fermenting the squid cartilage; 3)carrying out purification; 4) caryring out drying. Squid cartilage is used as a raw material, chondroitin sulfate lyase producing bacteria in small intestines of ducks are separated and applied to fermentation of the squid cartilage to prepare chondroitin sulfate glycoprotein, and a fermentation product is subjected to alcohol-salt primary purification, combined membrane-electric field secondarypurification and drying to obtain the product. The chondroitin sulfate extraction rate of the process is 98.7%. According to the method, a biological method and a combined purification method are adopted, the extraction rate is high, the biological activity is high, the prepared chondroitin sulfate is mainly chondroitin sulfate C, the technological process is simple, efficient and easy to control,and large-scale popularization and production are facilitated.

Description

technical field [0001] The invention relates to extracting glycoproteins from other marine animals, in particular to a method for preparing squid chondroitin sulfate glycoproteins. Background technique [0002] Squid processing is often accompanied by a large number of by-products, including cartilage, inner shell, etc. These wastes are rarely used, except for some processed into fish meal or feed, because they are perishable and deteriorated, they are treated as garbage, resulting in a waste of resources . Studies have shown that the main components of squid cartilage are protein and chondroitin sulfate, of which chondroitin is a biomedical product that has various activities such as lowering blood fat and preventing vascular sclerosis. Therefore, strengthening comprehensive utilization and turning waste into treasure can effectively increase its added value, reduce the cost of waste treatment, and promote the development of squid processing. Because squid cartilage has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C07K1/14
CPCC12P21/005C07K1/145
Inventor 吴文惠杨劲峰
Owner 荣成万盈水产科技有限公司
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