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Biosensor and method of distinguishing a light

A sensor and biological technology, applied in the direction of chemiluminescence/bioluminescence, material analysis through optical means, and analysis through chemical reaction of materials, etc., can solve complex and expensive manufacturing processes and other problems

Pending Publication Date: 2021-04-13
VISERA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, many efforts have been made to include light angle directing components on angle-sensitive filters to maintain small angles of light entering the filter to reduce spectral shift phenomena, which lead to complex and expensive manufacturing processes

Method used

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  • Biosensor and method of distinguishing a light
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  • Biosensor and method of distinguishing a light

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0107] In this embodiment, the biosensor 100 includes an angle sensitive filter 108 . Figure 7A The arrangement of analytes 122a on biosensor 100 is shown. Figure 7B show Figure 7A Sectional view along line A-A'. In this example, it is assumed that light emitted by an analyte reaches a pixel one pixel away from the pixel below the light. In other words, the closest eight pixels surrounding the pixel under this light. For example, if Figure 7A As shown, pixel 1041 is under the light emitted by analyte 122al, and pixels 1042-1049 are pixels that are only one pixel away from pixel 1041. Light emitted by analyte 122a1 may reach pixels 1041-1049.

[0108] Analyte 122a is placed on biosensor 100 . The area of ​​one analyte 122a is equal to or smaller than the area of ​​one pixel 104 .

[0109] In the case of fluorescence, excitation light 124 illuminates analyte 122a. The excitation light 124 can be moved from one side of the biosensor 100 to the opposite side so that th...

Embodiment approach 2

[0166] In this embodiment, the biosensor 200 includes an angle sensitive filter 108 and a first color filter 120a. Figure 9A The arrangement of analytes 122a on biosensor 200 is shown. Figure 9B show Figure 9A Sectional view along line A-A'. In this example, it is assumed that light emitted by an analyte reaches a pixel one pixel away from the pixel below the light. In other words, the closest eight pixels surrounding the pixel under this light. For example, if Figure 9A As shown, pixel 1041 is under the light emitted by analyte 122al, and pixels 1042-1049 are pixels that are only one pixel away from pixel 1041. Light emitted by analyte 122a1 may reach pixels 1041-1049.

[0167] Such as Figure 9A As shown, in the direction X, the first color filter 120a is separated by two pixels covered by the angle-sensitive filter 108, and in the direction Y perpendicular to the direction X, the first color filter 120a is separated by the angle The pixels covered by the sensitiv...

Embodiment approach 3

[0197] In this embodiment, the biosensor 300 includes an angle-sensitive filter 108, a first color filter 120a, and a second color filter 120b. Figure 11A The arrangement of analytes 122a on biosensor 300 is shown. Figure 11B show Figure 11A Sectional view along line A-A'. Figure 11C show Figure 11A Sectional view along line B-B'. Figure 11D show Figure 11A Sectional view along line C-C'. In this example, it is assumed that light emitted by an analyte reaches a pixel one pixel away from the pixel below the light. In other words, the closest eight pixels surrounding the pixel under this light. For example, if Figure 11A As shown, pixel 1041 is under the light emitted by analyte 122al, and pixels 1042-1049 are pixels that are only one pixel away from pixel 1041. Light emitted by analyte 122a1 may reach pixels 1041-1049.

[0198] Such as Figure 11A As shown, in the order of the first color filter / angle-sensitive filter / second color filter / angle-sensitive filter...

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Abstract

A biosensor and a method of distinguishing a light are provided. The biosensor includes a substrate, a first photodiode, a second photodiode, an angle-sensitive filter, and an immobilization layer. The first photodiode and the second photodiode are disposed in the substrate and define a first pixel and a second pixel, respectively. The first pixel and the second pixel receive a light. The angle-sensitive filter is disposed on the substrate. The immobilization layer is disposed on the angle-sensitive filter.

Description

technical field [0001] Embodiments of the present disclosure relate to a biosensor, in particular to a biosensor using a spectrum shifting layer for light detection. Background technique [0002] Recently, CMOS image sensors have also been used for biological or biochemical detection. For such applications, a biological or biochemical sample can be placed on the photodiode, and the light emitted by the biological or biochemical sample can be directed to the photodiode, and the fluorescence or chemiluminescent color of the sample can be detected and distinguished through the photodiode and strength. Different fluorescent molecules will produce different colors and intensities after being excited by light. Therefore, by judging the color and intensity of light received by the photodiode, it can be used to identify the interaction or characteristics of biological or biochemical samples on it. [0003] The general identification method of the color and intensity of light is to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/64
CPCG01N21/01G01N21/6402G01N21/6486G01N21/6454G01N21/76H01L27/14621H01L27/14629H01L27/14645G01N21/62
Inventor 谢馨仪谢锦全
Owner VISERA TECH CO LTD