Device and method for detecting human immunodeficiency virus based on neural network
A technology of AIDS virus and neural network, applied in the field of devices for detecting AIDS virus, which can solve the problems that the research on devices and methods for detecting AIDS virus has not been reported yet
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[0026] 1. Preparation of standard series of HIV-1-NL4-nano, HIV-1-NL4-EGFP virus and MLV virus
[0027] Accurately draw 200ul p24 concentration (34.4×10 6 pg / mL) HIV-1-NL4-nano virus solution, dilute to 6.9ml with serum to obtain 1x10 6 pg / ml HIV-1-NL4-nano virus stock solution, use 1x10 6 The HIV-1-NL4-nano virus stock solution and serum (as diluent) of pg / ml were respectively prepared according to 10-fold dilution, and 100ul virus solution was added to 900ul serum to obtain 1x10 5 , and so on to get 1x10 4 , 1x10 3 , 1x10 2 , 1x10 1 and 1x10 0 HIV-1-NL4-nano virus standard dilution in pg / ml.
[0028] Accurately draw 200ul p24 concentration (40.5×10 6 pg / mL) HIV-1-NL4-EGFP virus solution, dilute to 8.1ml with serum, get 1x10 6 pg / ml HIV-1-NL4-EGFP virus stock solution, use 1x10 6 The HIV-1-NL4-EGFP virus stock solution and serum (as diluent) of pg / ml were respectively prepared according to 10-fold dilution, and 100ul virus solution was added to 900ul serum to obtain...
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