The invention provides a universal method for detecting the total concentration of a
monoclonal antibody drug targeting soluble
protein in monkey serum. The method comprises the following steps: adding a universal coated
antibody working solution into an elisa plate, sealing the plate, and incubating overnight; adding the confining liquid into an elisa plate, sealing the plate, incubating, washing the plate with washing liquid, adding the diluted correction standard sample, blank monkey mixed serum, a
quality control product and a monkey serum sample to be detected into the elisa plate, sealing the plate, and incubating; washing the plate with washing liquor, adding the universal
enzyme-labeled detection
antibody working solution into the
enzyme-labeled plate, sealing the plate, and incubating; washing the plate with washing liquor, adding a substrate of
enzyme into the elisa plate, and incubating the elisa plate at
room temperature for color development; and adding
sulfuric acid into the elisa plate to stop color development, reading the
absorbance value of each hole of the elisa plate by using an elisa reader, and calculating the concentration value. The detection method provided by the invention can accurately and reliably determine the total
drug concentration of the
monoclonal antibody of the targeted soluble
protein in monkey serum and reflect the actual
blood drug concentration.