A method for rapid genetic transformation of peanut mediated by Agrobacterium

A genetic transformation method and technology mediated by Agrobacterium, applied in the field of rapid genetic transformation of peanut mediated by Agrobacterium, can solve the problems of limited genetic transformation of peanut, long transformation period, and difficulty in importing foreign genes.

Active Publication Date: 2021-09-10
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Peanut is a dicotyledonous plant and is one of the natural hosts of Agrobacterium. However, it is difficult to introduce foreign genes common to leguminous plants, which limits the research of peanut genetic transformation.
Although some progress has been made in the genetic transformation of peanuts in recent years, the current peanut genetic transformation system still has problems such as long transformation cycle and low transformation efficiency.

Method used

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  • A method for rapid genetic transformation of peanut mediated by Agrobacterium
  • A method for rapid genetic transformation of peanut mediated by Agrobacterium
  • A method for rapid genetic transformation of peanut mediated by Agrobacterium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1 Materials and methods

[0032] 1.1 Experimental materials and instrument reagents

[0033] 1.1.1 Plant materials tested

[0034] The tested peanut variety Ji Nonghua No. 1 in this study was provided by the Peanut Laboratory of Hebei Agricultural University.

[0035] 1.1.2 Experimental reagents

[0036] (1) culture medium

[0037] MS basic medium, YEB medium. The medium formula is shown in Table 1.

[0038] Table 1 medium formula

[0039]

[0040] (2) Test hormones, antibiotics and antioxidants

[0041] 6-benzylpurine (6-BA), a-naphthaleneacetic acid (NAA), gibberellin (GA 3 ), etodobutyric acid (IBA), kanamycin (Kan + ), cefotaxime (Cef + ), clavulanate carboxythiophene penicillin (Tim), acetosyringone (AS), streptomycin sulfate (Str), dithiothreitol (DTT), and the above mother liquor preparation methods are all shown in Table 2.

[0042] Table 2 Hormones, antibiotics and antioxidant mother solution preparation table

[0043]

[0044]

[0045] (3) ...

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Abstract

The invention discloses a method for rapid genetic transformation of peanuts mediated by Agrobacterium, and belongs to the technical field of peanut transgenesis. The present invention establishes a set of optimized system for Agrobacterium-mediated peanut half-seed genetic transformation technology, using peanut half-seed as explant, adding acetosyringone during infection and adding dithiothreitol during co-cultivation And adding 200mg / L asparagine and 200mg / L glutamine to each medium used in the culture process, so that the genetic transformation process can be completed within 5 weeks, greatly shortening the in vitro culture time and improving the transformation efficiency . The peanut rapid genetic transformation method provided by the invention can obtain transgenic plants more quickly, and lays a foundation for further utilizing genetic engineering to cultivate transgenic peanut varieties.

Description

technical field [0001] The invention relates to the technical field of peanut transgenesis, in particular to an Agrobacterium-mediated peanut rapid genetic transformation method. Background technique [0002] Peanut is an oilseed and economic crop widely planted in the world, rich in oil and protein. In the current production work, the breeding of peanut varieties is mainly carried out through conventional breeding methods, but the genetic basis of the varieties bred by traditional methods is narrow, and the excellent traits of wild peanut resources are difficult to use due to factors such as hybrid incompatibility. Genetic engineering technology can introduce exogenous genes into plants, artificially select genes for traits of interest, and achieve rapid improvement of varieties. Agrobacterium transformation is currently the most commonly used method in plant genetic engineering research, which has the advantages of low cost and low copy number of introduced genes. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84C12N1/20A01H5/00A01H6/54C12R1/01
CPCC07K14/415C12N1/20C12N15/8205
Inventor 刘立峰杨艳雯李秀坤崔顺立侯名语杨鑫雷刘盈茹赵楠楠邓洪涛
Owner HEBEI AGRICULTURAL UNIV.
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