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A selenium-resistant bacterial strain enterobacter ludwigii GX-C3 and its application

A strain, selenium-resistant technology, applied in the field of microbiology, can solve the problems of less microbial research

Active Publication Date: 2022-04-08
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, domestic research on selenium mainly focuses on the behavior of selenium in soil, selenium-enriched plants and nano-selenium materials, but less research on microorganisms in selenium-enriched soils. For this reason, the inventors collected soil samples from selenium-enriched areas in Guangxi. Identify the selenium-resistant strains and measure the removal rate of selenium-resistant strains, in order to provide strain resources for the development of selenium resources from the perspective of microorganisms

Method used

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  • A selenium-resistant bacterial strain enterobacter ludwigii GX-C3 and its application
  • A selenium-resistant bacterial strain enterobacter ludwigii GX-C3 and its application
  • A selenium-resistant bacterial strain enterobacter ludwigii GX-C3 and its application

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Embodiment

[0024] 1. Materials and methods

[0025] 1.1 Materials

[0026] 1.1.1 Bacteria screening soil

[0027] The bacteria screening soil was collected from four rice soil samples with different selenium content in Qintang District, Guigang City, Guangxi, and the four soils were named A, B, C, and D respectively. The soil to be tested was collected by the five-point mixing method to collect 0-20cm surface soil. After removing surface impurities, put it in a sterile ziplock bag and store it in an ice box. On the same day, it was brought back to the laboratory and stored in a 4°C refrigerator for later use. The physical and chemical properties of the soil are shown in the table 1.

[0028] Table 1 Soil physical and chemical properties and selenium content

[0029]

[0030] 1.1.2 Medium

[0031] Bacterial liquid medium: peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1L, pH 7.4-7.6.

[0032] Bacterial solid medium: peptone 10g, beef extract 3g, sodium chloride ...

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Abstract

The invention belongs to the technical field of microbiology, in particular to a selenium-resistant strain Enterobacter ludwigii GX-C3 and its application. A selenium-tolerant strain Enterobacter ludwigii GX-C3, whose taxonomic name is Enterobacter ludwigii, was deposited in the Guangdong Provincial Microbial Culture Collection Center on December 10, 2020, and the deposit number is GDMCC No.61358. The selenium-resistant strain Enterobacter ludwigii GX-C3 strain of the present invention has a maximum removal rate of 46.76% of selenium in soil at a selenium concentration of 2000 μg / mL. The results can provide bacterial resources for bioremediation in selenium-contaminated areas, and provide reference for the development and utilization of selenium resources.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and in particular relates to a selenium-resistant bacterial strain Enterobacter ludwigiiGX-C3 and an application thereof. Background technique [0002] Selenium is an essential trace element for the human body. Selenium deficiency will lead to an increase in the incidence of Keshan disease, cancer and cardiovascular diseases. Excessive selenium intake will cause hair loss and poisoning. Selenium mainly exists in four forms in nature, namely selenide (Se 2- ), elemental selenium (Se 0 ), selenite (SeO 3 2- ) and selenate (SeO 4 2- ), and the transformation of the valence state between various forms is closely related to microorganisms. The high-selenium environment is a potential source of selenium-tolerant microbial strains. Studies have shown that under high-selenium environmental stress, bacteria produce a stress response and convert highly toxic inorganic selenium into less toxic se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/01
CPCC09K17/14
Inventor 刘永贤申佩弘农梦玲黄婵婵潘丽萍廖青邢颖兰秀梁琪
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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