Induction medium and induction method for stem cell differentiation into sweat gland-like cells

Abstract

The invention discloses an induction medium for differentiating stem cells into sweat gland-like cells, and belongs to the technical field of stem cells. A induction culture medium is a DMEM / F-12 cell culture medium containing fetal bovine serum, an epidermal growth factor, a basic fibroblast growth factor, a recombinant human ectoderm dysplasia A1 protein, a recombinant human Wnt3a protein, a bone morphogenetic protein 4, a hepatocyte growth factor, triiodothyronine and hemisuccinyl hydrocortisone. The composition is prepared from insulin- transferrin-sodium selenite, L-glutamine and penicillin / streptomycin double antibiotics. The sweat gland induction culture medium can effectively induce stem cells, especially mesenchymal stem cells, to be differentiated into sweat gland-like cells with sweat gland-like cell phenotypes in vitro, the sweat gland-like cells can form a tubular structure similar to a sweat gland structure in glue, and the requirement for sweat glands in the treatment process of large-area burn patients can be met.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and in particular relates to an induction medium and an induction method for stem cells to differentiate into sweat gland-like cells. Background technique [0002] The skin is the largest organ in the human body and has functions such as protecting the body, perspiration, and sensing heat, cold, and pressure. Among them, sweat glands, as important functional skin appendages, can regulate body temperature, secrete sweat, and excrete some metabolites of the human body. Through the secretion and evaporation of sweat, sweat glands can discharge 25% of the body's heat to maintain a relatively constant body temperature. The number of sweat glands in the human body is certain, and its development is a complicated process. Once a large area is damaged, it will not be possible to quickly and effectively restore functional sweat glands. [0003] In mild burns, sweat gland-like cells can rely on the de...

AI Technical Summary

Problems solved by technology

The sweat gland-like cells were subjected to heat shock treatment, and the sweat gland-like cells were directly co-cultured with the mesenchymal stem cells after the heat shock, or the culture supernatant of the sweat gland-like cells after the heat shock was collected and co-cultured with the mesenchymal stem cells, and it was found that the mesenchymal stem cells could differentiate cells with sweat gland phenotype, but the composition of the conditioned medium in this method is unclear, the mechanism of differentiation is unknown, and it is uncertain whether functional sweat gland-like cells can be obtained

Images