Human and mammalian cell expression vector and system as well as construction method and application thereof

An expression vector, mammalian technology, applied in the field of genetic engineering, can solve the problems of influence interference, suboptimal transgene expression, the position of the expression vector, and the research on the adaptability of distance effect has not been reported.
CN112779289APending Publication Date: 2021-05-11XINXIANG MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XINXIANG MEDICAL UNIV
Publication Date
2021-05-11

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Abstract

The invention relates to a human and mammalian cell expression vector and system as well as a construction method and application thereof. The MAR1-68-Spacer-CMV-target gene-PolyA-MAR1-68 vector is constructed by inserting an MAR 1-68 sequence into the upstream of a promoter and the downstream of PolyA and inserting a segment of spacer DNA (Deoxyribonucleic Acid) sequence with the length of 500bp between the MAR sequence and the promoter at the same time. A Spacer segment in the vector is a neutral DNA sequence, and the transgenic expression level cannot be improved, so that the influence of a distance effect on transgenic expression can be really discussed. Experiments prove that the human and mammalian cell expression system containing the expression vector can significantly improve the expression level of carried target genes. Therefore, the mammalian cell expression vector and the expression system provided by the invention can overcome transgenic silencing, promote high-level stable expression of exogenous genes in host cells, and can be used for production of recombinant proteins.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a human and mammalian cell expression vector, an expression system, a construction method and application thereof. Background technique

[0002] At present, more than 70% of global therapeutic recombinant proteins are produced by human and mammalian cell expression systems, and expression vectors are one of the important factors for recombinant protein production. In the current human and mammalian cell expression systems, a problem that needs to be solved urgently is the phenomenon of transgene silencing. The phenomenon that the expression of foreign genes in the host is suppressed is called transgene silencing. The occurrence of transgene silencing is related to the position where the transgene is integrated into the host cell gene, that is, a "position effect". In addition, the strength of transgene expression is affected by the sequence of the vector ...

Claims

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