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Indoor biological assay method for green peach aphids

A bioassay and green peach aphid technology, which is applied in the fields of trapping or killing insects, applications, animal husbandry, etc., can solve problems such as abnormal death, affecting the accuracy of bioassay results, and high control mortality

Inactive Publication Date: 2021-05-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing method of indoor bioassay of green peach aphid is mainly the agar leaf disc method, but the leaf disc method has defects in the size of the leaf disc, twelve-hole plate and agar base, etc., resulting in high control mortality, escape of test insects and abnormal Phenomena such as death occur, affecting the accuracy of bioassay results

Method used

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  • Indoor biological assay method for green peach aphids
  • Indoor biological assay method for green peach aphids
  • Indoor biological assay method for green peach aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) plant uncoated cabbage, straight 3-4 true leaves, set aside;

[0034] (2) select the true leaves newly developed from the lower second layer, immerse in the dipropacyclopyrene solution for 15 seconds, the solvent of the solution is 0.05% TritonX-100 aqueous solution, take it out and ventilate for 2 hours;

[0035] (3) After the leaves are dried, insert 20-30 aphids without wings of uniform size (the number of aphids in different repeated tests is shown in Table 1);

[0036] (4) Use a self-made self-sealing bag gauze net bag (see figure 1 ) to cover the insect-receiving blade, and the port is reinforced and sealed with absorbent cotton (see figure 2 , image 3 ), record the infestation time;

[0037] (5) Mortality was checked after 72 hours.

[0038] In the above technical scheme, the selected cabbage leaves with uniform positions and sizes are dipped in insecticides first, and then the green peach aphid is inserted, which is closer to the feeding mode of the gr...

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Abstract

The invention relates to an indoor biological assay method for green peach aphids, and belongs to the technical field of plant protection. The indoor biological assay method comprises the following steps that a cruciferous plant which grows to 3-4 true leaves and is consistent in growth vigor is selected as a plant to be tested for biological assay, true leaves expanded from the lower second layer are uniformly selected for being soaked into a medicine to be tested, airing is carried out, wing-free green peach aphids are inoculated, insect receiving leaves are covered by using a gauze net bag modified by a self-sealing bag opening, the end opening is reinforced and sealed by using absorbent cotton, the insect inoculation time is recorded, the biological influence of the pesticide to be detected on the green peach aphid is judged by observing the death rate of the green peach aphids within a certain time, so that the indoor biological assay of the green peach aphids is achieved. The indoor biological assay method for the green peach aphids is good in air permeability and sufficient in space, survival of the green peach aphids in biological assay is facilitated, the influence of the space environment on the green peach aphids to be tested is reduced, and the accuracy of the biological assay is ensured.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to an indoor bioassay method for evaluating whether a substance to be tested affects the safety of peach aphids. Background technique [0002] Myzus Persicae, also known as smoke aphid, putty insect, belongs to Hemiptera (Hemiptera) Aphididae (Aphididae), is one of the polyphagous pests widely distributed all over the world. Excreted honeydew affects the photosynthesis process of the host and leads to the growth of pathogens, and also acts as a vector of plant viruses in various vegetable species. It has the characteristics of short reproductive cycle and large reproductive capacity. Under suitable conditions, it is very easy to break out in a short period of time, causing immeasurable losses to agricultural production. [0003] At present, the control of green peach aphid is mainly chemical control, and the timely spraying of chemical agents is still the main method at pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01M1/20
CPCA01K67/033A01M1/20
Inventor 高希武刘晓岚刘同先梁沛梁平卓
Owner CHINA AGRI UNIV
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