Construction method of wheat resisting dinitroaniline herbicide

Abstract

The invention discloses a construction method of wheat resisting dinitroaniline herbicide. The construction method comprises the following steps of: identifying a wheat tubulin gene, identifying a member with the highest homologous level with OsTubA2 in the wheat, identifying a potential effect site Met-212 of dinitroaniline, analyzing the conservation of adjacent sequences of the dinitroaniline, and evaluating the feasibility of a transformation scheme and editing of the dinitroaniline; and improving an adenine editor, creating an efficient WheiABE8e editor in the wheat, and efficiently editing the 212 site of the wheat tubulin gene by utilizing the WheiABE 8e editor. According to the construction method disclosed by the invention, a technical mechanism for quickly obtaining a wheat tubulin Met-212-Thr mutant so as to realize the effect of resisting the dinitroaniline herbicide is established by using WheiABE.

Description

technical field [0001] The invention belongs to the field of agricultural technology, in particular to a method for constructing dinitroaniline herbicide-resistant wheat. Background technique [0002] Weeds are a major farming challenge, limiting the yield and cost of crop cultivation. As a powerful weapon to suppress weeds, herbicide is a necessary part of modern agriculture, and herbicide-resistant crops are an effective means to improve the efficiency of herbicide use. Wheat, as one of the three major food crops in the world, is also deeply harmed by weeds. Screening herbicide-resistant germplasm is an important part of wheat breeding. Modifying the sensitive sites of herbicide-responsive proteins is a common strategy for cultivating herbicide-resistant germplasm. By modifying the 174 and 634 sites of the ALS gene in wheat, the sulfonylurea, imidazolinone and aryloxyphenoxy Resistance to propionic acid-type herbicides. [0003] Base editing technology is a new genetic ...

AI Technical Summary

Problems solved by technology

[0005] In addition, currently using the ABE7.10 editor for editing, the efficiency is low, and it can be applied to diploid crops such as rice. It is necessary to realize wheat ( Hexaploid, a gene has six alleles) saturation mutation of multiple alleles (simultaneous editing of multiple alleles) is more difficult

This will lead to increased breeding generations, extended R&D cycles, larger breeding groups, and increased breeding costs.

[0006] Through detection, no patent publications related to the patent application of the present invention have been found

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