Aquatic Biological Toxicity Experimental Device for Studying Organic Pollutants in Shallow Groundwater
A technology of organic pollutants and aquatic organisms, applied in the field of aquatic toxicity research, can solve problems such as inability to flexibly adjust experimental variables and restrict experimental research on aquatic toxicity, and achieve the effect of improving sealing performance
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[0045]1. The soil shallow groundwater collected from the dangerous goods warehouse workshop, the chlorinated paraffin workshop and three different areas outside the factory area are numbered A1, A2 and A3; the experimental organisms are Fisher Vibrio, artificially raised adult zebrafish and their embryos purchased from the Model Organism Research Center of Nanjing University;
[0046] 2. Put the three water samples A1, A2 and A3 into three experimental sample storage boxes 2 respectively, and mix Milli-Q ultrapure water and 60mg.L -1 The NaCl solution is placed in two nutrient solution storage cylinders 4 respectively, Milli-Q ultrapure water, 60mg.L -1 The NaCl solution and the three water samples A1, A2 and A3 are correspondingly put into three culture solution storage cylinders 4, and the culture solution of the required concentration is configured;
[0047] Three, Vibrio Fischer is cultivated on the cell culture plate 500 in the first experimental box 50, and zebrafish ad...
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