Pharmacological method of drug-containing liver incubation liquid and preparation method of drug-containing liver incubation liquid of ephedra, semen armeniacae amarae, gypsum and liquorice decoction

Abstract

The invention discloses a pharmacological method of drug-containing liver incubation liquid and a preparation method of the drug-containing liver incubation liquid of ephedra, semen armeniacae amarae, gypsum and liquorice decoction. The preparation method comprises the following steps of: processing orally-administrated traditional Chinese medicines according to a clinical method, taking decoction, obtaining drug-containing intestinal absorption liquid by adopting an everted enteric capsule method, taking the drug-containing intestinal absorption liquid and liver cell suspension for short-time incubation, and then preparing the drug-containing liver incubation liquid for pharmacological tests of in-vitro tissues and organs, cells and the like. The drug-containing liver incubation liquid prepared by the preparation method disclosed by the invention can reflect the actual migration or transformation status of chemical components of a traditional Chinese medicine compound entering a body more truly, has the advantages of strict and controllable conditions, less interference, high content of active ingredients, small standard deviation between groups and the like, and is used for in-vitro pharmacological research without the interference of endogenous components of serum, so that the drug-containing liver incubation liquid is high in stability and good in reproducibility and is more suitable for discussion of in-vitro pharmacological effects of the traditional Chinese medicine compound and mechanism thereof.

Description

technical field [0001] The invention relates to a new in vitro pharmacological research method of a compound traditional Chinese medicine, in particular to a pharmacological method of liver incubation solution containing medicine and a preparation method of liver incubation solution of Maxing Shigan Decoction. Background technique [0002] In vitro pharmacological research plays an important role in drug activity screening, toxicity evaluation, and in-depth cell and molecular pharmacology research. Western medicine is mostly a single compound, so after being dissolved in an appropriate solvent, in vitro pharmacological experiments can be directly carried out. Traditional Chinese medicine, especially the compound prescription, has many medicinal flavors and complex components. The direct addition method can interfere with the in vitro experiment due to the physical and chemical properties of the crude extract, such as impurities, pH, osmotic pressure, and pH value. The class...

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Problems solved by technology

Traditional Chinese medicine, especially the compound prescription, has many medicinal flavors and complex components. The direct addition method can interfere with the in vitro experiment due to the physical and chemical properties of the crude extract, such as impurities, pH, osmotic pressure, and pH value. The classic in vitro pharmacological research method of the compound is widely used in the discussion of its mechanism of action, material basis and compatibility theory. However, due to the complex system involved in serum, its stability, reproducibility, false positives and other problems are difficult to overcome. ; The pharmacological method of intestinal absorption liquid containing medicine has the advantages of simple operation, high content of ingredients, less interference, and good reproducibility. The whole process of intestinal absorption in the body and entering the blood through the portal vein through the liver is not completely consistent with the actual migration or transformation of Chinese medicine ingredients in the body; CSF pharmacology is the use of drug-containing cerebrospinal fluid instead of drug-containing serum for in vitro neuropharmacological studies of traditional Chinese medicine. Due to the small amount of cerebrospinal fluid and difficulty in obtaining it, it is limited to in vitro neuropharmacological studies

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