Sausage with Yeast-Soluble Beta-Glucan-Peanut Protein Isolate Complex
A technology for isolating peanut protein and glucan, which is applied in food science and other directions to achieve the effect of promoting water absorption
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Embodiment 1
[0028] A method for preparing sausages containing yeast soluble beta-glucan-peanut protein isolate complex, comprising:
[0029] (1) Yeast β-glucan was dissolved in deionized water to prepare a suspension with a mass fraction of 1.5%, and put it into a water bath at 45°C to preheat; add 4% helicase to the suspension, and preheat Heat for 5 minutes, shake and hydrolyze for 80 minutes; inactivate the enzymatic solution in a boiling water bath for 10 minutes, centrifuge to take the supernatant and freeze-dry it for later use to obtain yeast-soluble β-glucan;
[0030] (2) Dissolving peanut protein isolate powder in water with a mass ratio of 1:8, and then undergoing alkali dissolution, acid precipitation, and freeze-drying to obtain peanut protein isolate;
[0031] (3) Dissolve peanut protein isolate and yeast soluble β-glucan in phosphate buffer solution to obtain 10 mg / mL peanut protein isolate and 30 mg / mL yeast soluble β-glucan solution respectively, and yeast soluble β-glucan...
Embodiment 2
[0038] A method for preparing sausages containing yeast soluble beta-glucan-peanut protein isolate complex, comprising:
[0039] (1) Yeast β-glucan was dissolved in deionized water to prepare a suspension with a mass fraction of 1.5%, and put it into a water bath at 45°C to preheat; add 4% helicase to the suspension, and preheat Heat for 5 minutes, shake and hydrolyze for 80 minutes; inactivate the enzymatic solution in a boiling water bath for 10 minutes, centrifuge to take the supernatant and freeze-dry it for later use to obtain yeast-soluble β-glucan;
[0040] (2) dissolving the peanut protein isolate powder in water with a mass ratio of 1:12, and undergoing alkali dissolution, acid precipitation, and freeze-drying to obtain the peanut protein isolate;
[0041](3) Dissolve peanut protein isolate and yeast soluble β-glucan in phosphate buffer solution to obtain 2 mg / mL peanut protein isolate and 6 mg / mL yeast soluble β-glucan solution respectively; yeast soluble β-glucan T...
Embodiment 3
[0048] A method for preparing sausages containing yeast soluble beta-glucan-peanut protein isolate complex, comprising:
[0049] (1) Yeast β-glucan was dissolved in deionized water to prepare a suspension with a mass fraction of 1.5%, and put it into a water bath at 45°C to preheat; add 4% helicase to the suspension, and preheat Heat for 5 minutes, shake and hydrolyze for 80 minutes; inactivate the enzymatic solution in a boiling water bath for 10 minutes, centrifuge to take the supernatant and freeze-dry it for later use to obtain yeast-soluble β-glucan;
[0050] (2) Dissolving peanut protein isolate powder in water with a mass ratio of 1:8, and then undergoing alkali dissolution, acid precipitation, and freeze-drying to obtain peanut protein isolate;
[0051] (3) Dissolve soybean protein isolate and yeast soluble β-glucan in phosphate buffer solution to obtain 2mg / mL soybean protein isolate and 6mg / mL yeast soluble β-glucan solution respectively; yeast soluble β-glucan Mix ...
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