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Culturing container, culturing method, and transporting method

A culture container and container body technology, applied in the field of high efficiency, can solve problems such as slowing down the flow rate of the medium, inappropriate long-term cultivation, and difficulty in obtaining spheres of the desired size and shape.

Pending Publication Date: 2021-06-08
TOYO SEIKAN GRP HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In mass culture of spheroids using such a culture container, there is a problem that spheroids escape from the well (recess) and move to other wells, making it difficult to obtain spheroids of desired size and shape.
[0004] For example, in order to carry out medium exchange, when liquid culture medium is provided in the culture bag (bag-shaped culture container) that has many holes on the culture surface, the spheroid is easy to run out from the hole and move to other wells, therefore, Requires extremely slow medium flow rate, rapid medium exchange is very difficult
Therefore, such culture vessels are not suitable for long-term cultures that require frequent exchange of media
[0005] In addition, in such a culture container, since the spheres easily escape from the hole even if only vibration is applied, it is not easy to move the culture container safely during the culture of the spheres.

Method used

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  • Culturing container, culturing method, and transporting method
  • Culturing container, culturing method, and transporting method
  • Culturing container, culturing method, and transporting method

Examples

Experimental program
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no. 1 approach

[0035] refer to figure 1 and figure 2 The culture container, culture method, and transportation method according to the first embodiment of the present invention will be described. figure 1 In order to show the top plate and the schematic diagram of the container main body in the culture container which concerns on this embodiment, figure 2 It is a diagram showing a schematic plan view and a cross-sectional view obtained by enlarging a part of the culture vessel according to the present embodiment.

[0036] The culture container of the present embodiment is a culture container provided with a plurality of recesses for accommodating objects to be cultured. figure 1 The illustrated container body 10 and top plate 20 are bonded together by heat sealing or the like and formed.

[0037] The container main body 10 has a first surface 10-1 on which the opening 12 in the recess 11 is formed, and the bottom of the culture container is formed by using the surface forming the first ...

no. 2 approach

[0094] In the following, refer to Figure 5 The culture container, culture method, and transportation method according to the second embodiment of the present invention will be described. Figure 5 It is a diagram showing a schematic plan view and a cross-sectional view obtained by enlarging a part of the culture vessel according to the present embodiment.

[0095] The culture container of the present embodiment is provided with a plurality of recesses for accommodating culture objects, such as Figure 5 As shown, a container main body 10b and a top plate 20b are provided.

[0096] The container main body 10b has a first surface 10b-1 on which the opening 12b in the recess 11b is formed, and the surface forming the first surface 10b-1 and the side walls of the recess 11b constitutes the bottom of the culture container as a culture surface.

[0097] The top plate 20b has a second surface 20b-1 opposite to the first surface 10b-1 of the container main body 10b.

[0098] That ...

no. 3 approach

[0109] refer to Image 6 The culture container, culture method, and transportation method according to the third embodiment of the present invention will be described. Image 6 It is a diagram showing a schematic plan view and a cross-sectional view obtained by enlarging a part of the culture vessel according to the present embodiment.

[0110] The culture container of the present embodiment is provided with a plurality of recesses for accommodating culture objects, such as Image 6 As shown, a container main body 10c and a top plate 20c are provided.

[0111] The container main body 10c has a first surface 10c-1 on which the opening 12c in the recess 11c is formed, and the surface forming the first surface 10c-1 and the side walls of the recess 11c constitutes the bottom of the culture container as a culture surface.

[0112] The top plate 20c has a second surface 20c-1 opposite to the first surface 10c-1 of the container main body 10c. exist Figure 4 , some of these fac...

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Abstract

In a culturing container having a plurality of recess sections formed to culture culturing target objects such as spheres, liquid objects can communicate between the recess sections and the movement of the culturing target objects between the recess sections can be suppressed. This culturing container, which comprises a plurality of recess sections (11) for storing culturing target objects, is provided with: a container boy (10) having a first surface (10-1) having the recess sections formed therein; and a top plate (20) having a second surface (20-1) facing the first surface, wherein protrusion sections and / or flow path sections are provided on the first surface side and / or the second surface side, a flow path is formed between the container body and the top plate through the contact between the container main body and a portion (21) of the top plate, and the width of the flow path is smaller than the minimum diameter of the culturing target objects.

Description

technical field [0001] The present invention relates to techniques related to cell culture, and specifically relates to techniques for improving the efficiency of production of spheroids and the like. Background technique [0002] In recent years, when culturing large amounts of adherent cells such as stem cells such as iPS cells and ES cells, not only the cells are allowed to adhere to the culture container to proliferate, but also the cells coated with a low-adhesive substrate have been used. A method of culturing cells in a three-dimensional state closer to the living body by forming spheroids (spheroids, agglomerates) in culture containers such as microwell plates. [0003] In mass culture of spheroids using such a culture container, there is a problem that spheroids escape from the well (recess) and move to other wells, making it difficult to obtain spheroids of desired size and shape. [0004] For example, in order to carry out medium exchange, when liquid culture med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/07
CPCC12M23/12C12M23/16C12M23/38C12M25/06C12M41/32
Inventor 小关修田中乡史
Owner TOYO SEIKAN GRP HLDG LTD