Bovine-derived pathogenic bacillus cereus indirect ELISA detection kit

The technology of Bacillus cereus and detection kit is applied in the field of microbial detection to achieve the effects of high sensitivity, fast detection process and strong detection specificity

Pending Publication Date: 2021-06-18
INNER MONGOLIA UNIV FOR THE NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But before this, there was no relevant report about the infection of the bacteria to the death of cattle.

Method used

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  • Bovine-derived pathogenic bacillus cereus indirect ELISA detection kit
  • Bovine-derived pathogenic bacillus cereus indirect ELISA detection kit
  • Bovine-derived pathogenic bacillus cereus indirect ELISA detection kit

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Embodiment Construction

[0023] The indirect ELISA detection kit of the present invention uses bovine-derived pathogenic Bacillus cereus thalline as the coated antigen, and by determining the concentration of the coated antigen, the dilution of the serum to be tested, and the critical value of the positive serum and the negative serum, the final Establish and assemble the bovine-derived pathogenic Bacillus cereus indirect ELISA detection kit.

[0024] 1. Optimization of indirect ELISA detection method:

[0025] Take the standard positive serum and negative serum, and use the purified and cultured Bacillus cereus cells as the coating antigen to conduct orthogonal experiments to optimize the reaction conditions of indirect ELISA: set the antigen coating concentration gradient respectively, that is, the dilution concentration of the bacterial solution is 1 / 20, 1 / 40, 1 / 60, 1 / 80, and 1 / 100, coated overnight at 4°C; serum dilutions were 1 / 50, 1 / 100, 1 / 200, and 1 / 400, incubated at 37°C for 1 hour; blocked ...

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Abstract

The invention relates to a microbiological detection technology, in particular to a bovine-derived pathogenic bacillus cereus indirect ELISA detection kit. The indirect ELISA detection kit is characterized in that the indirect ELISA detection kit takes a bovine-derived pathogenic bacillus cereus suspension as a coating antigen. The indirect ELISA detection kit has the beneficial effects that the prepared detection kit is suitable for detecting bovine-derived pathogenic bacillus cereus, has the characteristics of strong detection specificity, high sensitivity, fast detection process, convenience in operation and the like, and provides an effective technology and tool for preventing bovine bacillus cereus infection.

Description

technical field [0001] The invention relates to microorganism detection technology, in particular to an indirect ELISA detection kit for bovine-derived pathogenic Bacillus cereus. Background technique [0002] Bacillus cereus (Bacillus cereus. Frankland) belongs to the Bacillus family and belongs to the genus Bacillus cereus. This bacterium has similar biological characteristics to Bacillus thuringiensis and Bacillus anthracis, and it widely exists in the natural environment and food. It can produce antibacterial substances, inhibit the reproduction of harmful microorganisms, degrade the nutrients in the soil, and improve the ecological environment. Some Bacillus cereus can be used as plant growth promoters and microbial additives in animal feed. The bacterium is an opportunistic pathogenic bacterium, and it often causes vomiting or diarrhea in humans or animals in clinical practice. It is one of the common foodborne pathogenic bacteria. But before this, there was no relev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/68C12N1/20C12R1/085
CPCG01N33/56911G01N33/54393G01N33/6854C12N1/20G01N2333/32G01N2469/20
Inventor 王学理何云江郗珊珊孟庆磊陈云娇
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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