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Application of Bexarotene in preparation of medicine for resisting pituitary adrenocorticotropic hormone adenoma

A technology of adrenal cortex and pituitary gland, applied in the field of medicine, to achieve the effect of reducing mRNA expression level, lowering ACTH level, and inhibiting tumor growth

Pending Publication Date: 2021-06-22
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The role of bexarotene in the preparation of anti-pituitary corticotropic adenoma drugs has not been reported

Method used

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  • Application of Bexarotene in preparation of medicine for resisting pituitary adrenocorticotropic hormone adenoma
  • Application of Bexarotene in preparation of medicine for resisting pituitary adrenocorticotropic hormone adenoma
  • Application of Bexarotene in preparation of medicine for resisting pituitary adrenocorticotropic hormone adenoma

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0040] Experimental Experiment 1. Effect of Saridin on proliferation of mouse pituitary tumor cells (ACTH secretion type, ATT-20 cells).

[0041] Experimental materials: ATT-20 cells were purchased from American Type CultureCollection. Boussarodin was purchased from SELLECK (China, Shanghai Bluemum Chemical Co., Ltd.). Dimethyl sulfoxide (DMSO) is purchased from Merck Life Sciences (Nantong) Co., Ltd. DMEM, fetal bovine serum (FBS) and Horse serum (HS) were purchased from Saimo Fel Technology (China) Co., Ltd. Pyramomycin mixture is purchased from Beijing Solabao Technology Co., Ltd. CCK-8 doses were purchased from Jiangsu Kaizi Biotechnology Co., Ltd.

[0042] Solution is formulated: Saassolin is dissolved in DMSO to a 5 mm concentration of 100 mm.

[0043] ATT-20 cells were inoculated at 96-well plates, 0.5% FBS hunger cells were added to FBS to a final concentration of 10%, and the dose of the final concentration 0, 10, 20, 40, 60, 80, 100 um was added to the Saar Luo, cultured...

experiment example 2

[0048] Experimental Example 2. Effects of Saridin on tumor growth in mice in the skinned tumor model.

[0049] Experimental materials: BAL / BC mice were purchased from Beijing Wirong Lihua Experimental Animal Technology Co., Ltd. ATT-20 cells were purchased from American Type Culture Collection. Boussarosine, timomazolelamine and Gekinib were purchased from SELLECK (China, Shanghai Bluemum Chemical Co., Ltd.). Carboxymethylcellulose (CMC-NA) is purchased from National Medicine Group Chemical Reagent Co., Ltd.

[0050] Modeling group: 16-18g of female mice after hair removal of ATT-20 cells, 5 days after the tumor growth is 3-5mm in diameter, randomly divided into model group (1% CMC-NA), teloxazole group (20 mg · kg -1 , Positive controls), Gefitinib group (75mg · kg -1 , Negative controls) and Bunar Lotin group (50mg · kg -1 8. Each group, normal mouse with uncharged tumors.

[0051] 1% carboxymethylcellulose sodium is dissolved, boiled, and then cooled as a solvent, and the Saa...

experiment example 3

[0055] Experimental Example 3. Effect of Saridolin to the level of blood pulp adrenocortic hormone (ACTH) of mice in the skinned tumoroma model.

[0056] Experimental materials: BAL / BC mice were purchased from Beijing Wirong Lihua Experimental Animal Technology Co., Ltd. ATT-20 cells were purchased from American Type Culture Collection. Boussarosine, timomazolelamine and Gekinib were purchased from SELLECK (China, Shanghai Bluemum Chemical Co., Ltd.). Carboxymethylcellulose (CMC-NA) is purchased from National Medicine Group Chemical Reagent Co., Ltd. Mouse ACTH ELISA reagent boxes were purchased in Wuhan Huamei Biological Engineering Co., Ltd.

[0057] Experimental grouping: related experiment groups and administration treated with Experimental Example 2.

[0058] The end of the administration is taken from the eye, immediately low temperature, and detects the mouse ACTH ELISA kit, the steps are as follows:

[0059] The kit was shifted from 4 degrees to room temperature for 20 m...

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Abstract

The invention discloses an application of Bexarotene in preparation of a medicine for resisting pituitary adrenocorticotropic hormone adenoma, the pituitary adrenocorticotropic hormone adenoma enables a patient to excessively secrete corticotropin (ACTH) for a long time, and the adrenal gland is stimulated to secrete a large amount of cortisol, so that symptoms such as centripetal obesity, osteoporosis, amyotrophy, sexual dysfunction, hypertension, insomnia and the like of patients, which belong to rare diseases, can be caused. The Bexarotene can significantly inhibit tumor growth and inhibit ACTH secretion and expression of a precursor POMC of ACTH. The Bexarotene can be used for preparing the medicine for resisting pituitary adrenocorticotropic hormone adenoma, adverse reactions are reduced, and a safe, effective and economical solution is provided for treatment and prevention of diseases.

Description

Technical field [0001] The present invention relates to the application of the preparation of drugs in the preparation of drugs, mainly involving the application of the preparation of anti-pituitary academic adrenocorticoid adenoma drugs, belonging to the technical field of medical technology. Background technique [0002] Pituitary academic adrenal hormone adenoma is a benign hormone secretory tumor that occurs in front of the pituitary, which is a rare disease. It is the main cause of Kutin disease. [0003] Pituitary academic adrenocorticoid adenoma causes the patient's pituitary pituitary leaf long-term secretion of ACTH, stimulates the adrenal cortex to produce excess cortisol, and the clinical manifestation is Kukin syndrome, the pathological changes include, but is not limited to, to the heart, buffalo, hair, hair increase, hair increase, Skin muscle atrophy, nervous system injury, hypertension, atherosclerosis, diabetes, immune injury and sexual dysfunction. [0004] Pitu...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61P35/00A61P5/38A23L33/00
CPCA61K31/192A61P35/00A61P5/38A23L33/00A23V2002/00A23V2200/308
Inventor 孙岚杜冠华于浩滢吕扬任淑月王景蓉吕婷婷
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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