Visual virus detection method based on CRISPR-Cas12a system and application

A virus detection, coronavirus technology, applied in biochemical equipment and methods, microbial determination/inspection, resistance to vector-borne diseases, etc., can solve problems such as undiscovered patent publications, and achieve excellent reliability and accurate detection methods. , Easy to operate effect

Pending Publication Date: 2021-06-25
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Visual virus detection method based on CRISPR-Cas12a system and application
  • Visual virus detection method based on CRISPR-Cas12a system and application
  • Visual virus detection method based on CRISPR-Cas12a system and application

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Embodiment Construction

[0048] Below in conjunction with embodiment, the present invention is further described, and following embodiment is descriptive, not limiting, can not limit protection scope of the present invention with following embodiment.

[0049] The raw materials used in the present invention, if no special instructions, are conventional commercially available products, the methods used in the present invention, if no special instructions, are conventional methods in this area, and the quality of each material used in the present invention is routine use quality.

[0050] The experimental methods in the examples of the present invention that do not specify specific conditions are usually carried out according to conventional conditions, such as the conditions described in "Molecular Cloning: A Laboratory Manual" (New York Cold Spring Harbor Laboratory Press, 1989). The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specifie...

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Abstract

The invention discloses a visual virus detection method based on a CRISPR-Cas12a system. The method comprises the following steps: carrying out reverse transcription on a virus genome or directly carrying out PCR amplification to obtain dsDNA, namely target DNA; and designing crRNA of a specific targeting virus gene. When target DNA exists, Cas12a-crRNA specifically recognizes target DNA, activates the activity of Cas12a non-specific ssDNase, degrades linker ssDNA connected with an AuNPs-DNA probe, induces the change of the aggregation-dispersion state of the AuNPs-DNA, and the change of the aggregation-dispersion state of nanogold generates obvious color change. The color change can be directly judged through naked eyes, and the detection result can be read and analyzed through a smart phone App color recognizer.

Description

technical field [0001] The invention belongs to the technical field of virus detection, in particular to a visual virus detection method and application based on a CRISPR-Cas12a system. Background technique [0002] Virus infection is a serious threat to human health. Virus detection is crucial to the diagnosis, prevention and treatment of diseases. The development of effective, accurate, rapid and sensitive detection tools plays a vital role in disease control. Although the development of detection methods in the laboratory has made great progress, it is still necessary to strengthen the development of detection methods under non-laboratory conditions, especially to promote the field detection (point-of-care testing, POCT). applicability. CRISPR-Cas-based nucleic acid detection technology provides a novel way to develop sensitive and rapid POCT detection. At present, virus detection methods mainly include nucleic acid detection, antibody detection, and antigen detection. ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12Q1/682
CPCC12Q1/701C12Q1/686C12Q1/682C12Q2563/107C12Q2521/327C12Q2563/155Y02A50/30
Inventor 马龙殷利眷李晓燕陈思满淑丽
Owner TIANJIN UNIV OF SCI & TECH
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