Mitochondrial membrane potential detection kit

Abstract

The invention discloses a mitochondrial membrane potential detection kit which comprises an outer kit body and an inner kit body. Heaters are arranged on the inner side wall of the bottom of the inner kit body. A vacuum bin and a heating bin are arranged in the outer kit body. Fixing grooves are formed in the two ends of the inner side wall of the inner kit body, a plurality of magnets are fixedly connected to the inner side walls of the fixing grooves, and sliding grooves are formed in the inner walls of the fixing grooves. A plurality of testing plates are arranged in the inner kit body, and five staining culture media are arranged on the upper side wall of each testing plate. Limiting mechanisms are arranged on the outer side wall of the inner kit body, and a reset mechanism is arranged at the top of the vacuum bin. The two ends of the testing plates slide up and down along the inner side walls of the sliding grooves. According to the mitochondrial membrane potential detection kit, by arranging the heaters, the testing plates and the staining culture media, the sterile culture of cells is realized; and under the heating action of the heaters, a high-temperature sterilization effect on the culture environment of the cells in the staining culture media in the inner kit body is achieved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a mitochondrial membrane potential detection kit. Background technique [0002] Mitochondria are moving plant cell The main place where ATP is generated is an important organelle that promotes cell energy conversion and participates in cell apoptosis; when mitochondria generate energy, they store electrochemical potential energy in inner mitochondrial membrane , on both sides of the inner membrane, if the asymmetric distribution of proton and other ion concentrations will form the mitochondrial membrane potential. [0003] The utility model with the patent application number CN203658263U discloses a mitochondrial membrane potential detection kit, including a box body, a box cover, a reagent liner, a JC-1 fluorescent probe, ultrapure water, a JC-1 staining buffer, and a CCCP inhibitor The box body is made of hard plastic, and a reagent liner made of hard plastic fo...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the shortcomings of the neglect of the cell culture environment in the prior art, which leads to cell inactivation, and then causes the failure of the mitochondrial membrane potential detection test, and proposes a mitochondrial membrane potential detection kit

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