Rapid extraction device for extracellular vesicles

An extraction device and cell technology, applied in the field of medical devices, can solve the problems of low extraction efficiency, insufficient purity, insufficient convenience and speed, etc., and achieve the effects of speeding up precipitation, improving sensitivity, and improving efficiency

Active Publication Date: 2021-07-09
SUZHOU AIBAODE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention overcomes the deficiencies of the prior art and provides a rapid extraction device for extracellular vesicles, aiming to solve the problems of low extraction efficiency, insufficient purity, and insufficient convenience and speed in the existing extracellular vesicle extraction methods

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  • Rapid extraction device for extracellular vesicles
  • Rapid extraction device for extracellular vesicles
  • Rapid extraction device for extracellular vesicles

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Embodiment Construction

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0037] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention can also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited by the specific implementation disclosed below. example limitations.

[0038] In the description of this application, it should b...

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Abstract

The invention discloses a rapid extraction device for extracellular vesicles. The rapid extraction device comprises a precipitation assembly, a ring hoisting device used for being connected with the precipitation assembly and a chromatography assembly connected with the precipitation assembly in a sleeving or threaded mode; a to-be-filtered part and a plurality of filters are arranged in the precipitation assembly, and a to-be-filtered solution containing extracellular vesicles is arranged in the to-be-filtered part; the ring hoisting device comprises a bracket, rotary generators and clamping rings matched with the corresponding rotary generators, and the clamping rings are used for clamping the precipitation assembly; the rotation directions and the rotation speeds of the adjacent clamping rings are kept consistent or inconsistent, and each rotation generator can enable the corresponding clamping ring to drive the precipitation assembly to rotate; a mixing part, and a plurality of first chromatographic columns and a plurality of second chromatographic columns which are connected with the bottom of the mixing part are arranged in the chromatographic assembly; and at least two first chromatographic columns are connected in series with the second chromatographic column. By combining a polymer precipitation method and a chromatography method, the problems that the extracellular vesicles are not high in extraction efficiency, not enough in purity and not convenient and rapid enough are solved.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a rapid extraction device for extracellular vesicles. Background technique [0002] Extracellular vesicles refer to vesicle-like bodies with a double-membrane structure shed from the cell membrane or secreted by cells, with diameters ranging from 40 nm to 1000 nm. Extracellular vesicles are mainly composed of microvesicles and exosomes. Microvesicles are small vesicles shed from the cell membrane after cell activation, injury or apoptosis, with a diameter of about 100nm-1000nm. Extracellular vesicles widely exist in cell culture supernatant and various body fluids (blood, lymph, saliva, urine, semen, milk), and carry a variety of proteins, lipids, DNA, mRNA, miRNA related to cell origin It is involved in processes such as intercellular communication, cell migration, angiogenesis and immune regulation. Elevated levels of extracellular vesicles are found in diabetes, card...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/46
CPCG01N30/06G01N30/461G01N2030/062
Inventor 徐锐
Owner SUZHOU AIBAODE BIOTECHNOLOGY CO LTD
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