Rice seedling strengthening agent and preparation method thereof
A seedling-strengthening agent and rice technology, which is applied in the agricultural field, can solve the problems of cultivating strong seedlings, uneven effects of disease and stress resistance, and a single component of the seedling-strengthening agent, so as to improve soil health, speed up the process of cell division, and improve The effect of soil vitality
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Embodiment 1
[0042] The preparation method of rice seedling strengthening agent is as follows:
[0043] 1) Cultivation of Trichoderma harzianum: Inoculate Trichoderma harzianum into PDA medium, culture at 28°C for 3-5 days, collect spores; inoculate spores on wheat grains, cultivate at 28°C for 7 days, dry in the shade naturally, and crush wheat spare grains;
[0044]2) Cultivation of Bacillus subtilis: 3 g of yeast powder, 5 g of sodium carboxymethyl cellulose, 10 g of peptone, 2.5 g of ammonium sulfate, 5 g of sodium chloride, 2 g of dipotassium hydrogen phosphate, and constant volume of 1 L. After sterilization, take the inoculated purified Bacillus subtilis into a 500ml Erlenmeyer flask filled with sterile 200ml sporulation medium, culture at 30°C for 30h at 200rpm / min;
[0045] 3) Cultivation of Bacillus licheniformis: 5 g of beef extract, 5 g of yeast extract, 10 g of peptone, 2.5 g of ammonium sulfate, 5 g of sodium chloride, pH 7.5, and constant volume of 1 L were used to make a s...
Embodiment 2
[0052] The specific operation steps are the same as in Example 1, except that Trichoderma harzianum, Bacillus licheniformis and Bacillus subtilis are fully mixed according to the mass ratio of 1:0.5:1.5.
Embodiment 3
[0054] The specific operation steps are the same as in Example 1, except that the Trichoderma harzianum, Bacillus licheniformis and Bacillus subtilis are fully mixed according to the mass ratio of 1:0.7:1.2, and the composite microbial agent is added according to the mass ratio of 10wt%.
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