UPLC determination of rhubarb anthraquinones in compound gentian sodium bicarbonate tablet
A technology of sodium bicarbonate tablets and anthraquinones, which is applied in the field of medical testing, can solve the problems of not being able to meet the work requirements, and can not measure energy at the same time, and achieve the effects of being suitable for large-scale promotion and use, simple measurement methods, and convenient operation
Pending Publication Date: 2021-07-20
青海省药品检验检测院
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Genuine rhubarb mainly contains anthraquinone derivatives, including free anthraquinones such as emodin, chrysophanol, aloe-emodin, emodin methyl ether, and rhein, and dianthrone glycoside-bound anthraquinones such as sennoside A and B. Studies have found that anthraquinones in rhubarb extract can regulate intestinal peristalsis and cause diarrhea. The HPLC method currently used for determination of compound gentian sodium bicarbonate tablets cannot detect rhubarb glycosides in compound gentian sodium bicarbonate tablets. , Rhubarb total anthraquinones and free anthraquinones are measured at the same time, and the determination method has limitations and cannot meet the work requirements
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[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on The embodiments of the present invention and all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0021] see Figure 1-4 , the present invention provides a technical solution:
[0022] A kind of UPLC assay of rhubarb anthraquinones in compound gentian sodium bicarbonate tablet, its specific steps are as follows:
[0023] S1, preparation of the reference substance solution Take the appropriate amount of six reference substances of rhubarb, emodin, rhein, chrysophanol, aloe-emodin, and emodin methyl ether, add a small amount of chloroform to ...
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The invention relates to the technical field of medicine detection, in particular to UPLC (Ultra Performance Liquid Chromatography) determination of rhubarb anthraquinones in compound gentian sodium bicarbonate tablets, which comprises the following specific steps: S1, preparing a reference substance solution, taking a proper amount of six reference substances, namely borrhein, emodin, rhein, chrysophanol, aloe-emodin and physcion, adding a small amount of chloroform for dissolving, and diluting the solution with methanol to make a solution containing about 0.1mg per 1ml as a concentrated solution of the reference substance. According to the method, an HPLC (High Performance Liquid Chromatography) method is converted into an UPLC (Ultra Performance Liquid Chromatography) method through design, the amount of total anthraquinone and free anthraquinone in rheum officinale is measured while rhaponticin in the compound gentian sodium bicarbonate tablet is screened, The content of rhaponticin and rhubarb anthraquinones in the compound gentian sodium bicarbonate tablet can be effectively and accurately detected, the determination method is simple and convenient to operate, and multiple components contained in the compound gentian sodium bicarbonate tablet are determined, so that different working requirements can be met, the use performance is higher, and the compound gentian sodium bicarbonate tablet is more suitable for large-scale popularization and application.
Description
technical field [0001] The invention relates to the technical field of medical detection, in particular to UPLC determination of rhubarb anthraquinones in compound gentian sodium bicarbonate tablets. Background technique [0002] The compound gentian sodium bicarbonate tablet is a compound preparation composed of sodium bicarbonate, rhubarb paste (powder), gentian paste (powder), clove oil, peppermint oil and suitable auxiliary materials. The rhubarb in the prescription should come from the Polygonaceae plants Rheum palmatum, Rhubarb tangutica or medicinal rhubarb, and does not contain rhubarb, while counterfeit products such as Zangbian rhubarb, North China rhubarb and Boye rhubarb contain rhubarb. Genuine rhubarb mainly contains anthraquinone derivatives, including free anthraquinones such as emodin, chrysophanol, aloe-emodin, emodin methyl ether, and rhein, and dianthrone glycoside-bound anthraquinones such as sennoside A and B. Studies have found that anthraquinones in ...
Claims
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Login to View More IPC IPC(8): G01N30/02G01N30/74G01N30/06G01N30/14G01N30/34
CPCG01N30/02G01N30/74G01N30/06G01N30/14G01N30/34G01N2030/146
Inventor陈学艳魏文芝张敏娟海平郑永彪袁璐张耀元阿玉梅吕蓓蓓彭双杨海源唐荣段广佩张幸福宋小利程华孔祥兰苏延菊王雯慧保敏敏
Owner青海省药品检验检测院