Primer combination and method for detecting common respiratory viruses

Abstract

The invention discloses a primer combination and a method for detecting common respiratory viruses. The primer combination comprises two or more of 12 pairs of primers for detecting 15 common respiratory viruses. The detection method comprises the following steps: performing high-throughput sequencing on a captured sequence of a to-be-detected sample, and comparing the sequence with characteristic sequences of specific region nucleic acids of 15 common respiratory viruses by using a bioinformatics analysis method, so that whether the sequence of the to-be-detected sample is matched with the 15 common viruses or not can be very accurately judged. The primer combination has the advantages of high accuracy and high flux.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and more specifically, the invention relates to a combination of primers and a method for detecting 15 common respiratory viruses. Background technique [0002] Respiratory viruses refer to viruses that mainly invade the respiratory tract, proliferate in the epithelial cells of the respiratory tract mucosa, and cause local infection of the respiratory tract or lesions of tissues and organs other than the respiratory tract, including common influenza viruses, human metapneumoviruses, syncytial viruses, parainfluenza viruses, and coronaviruses Wait. According to statistics, clinically about 90% to 95% of acute respiratory infections are caused by such respiratory viruses. [0003] In recent years, new pathogenic respiratory viruses have been continuously discovered, such as metapneumovirus, coronavirus, boca virus, etc., which pose a great threat to human health. Most acute respiratory inf...

AI Technical Summary

Problems solved by technology

However, the symptoms of infection caused by respiratory viruses are often similar, and it is difficult to make a definite diagnosis based on clinical symptoms and routine detection methods.

[0004] At present, the methods commonly used to detect respiratory viruses are fluorescent quantitative PCR, etc. These methods cannot obtain the sequence of the virus. If the virus undergoes a mutation of a few bases, it may lead to missed detection such as the disappearance of the fluorescent signal.

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