Primer combination and method for detecting common respiratory viruses
A primer combination and detection method technology, applied in biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., can solve problems such as disappearance of fluorescent signal, inability to obtain virus sequence, similarity, etc.
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Embodiment 1
[0049] The detection method of embodiment 1 common respiratory virus
[0050] Please refer to figure 1 , is a flow chart of the method for detecting common respiratory viruses in this embodiment, comprising the following steps:
[0051] 1. Sample pretreatment
[0052] In this embodiment, a total of 253 throat test sub-samples with known clinical results were selected for testing, 233 of which were samples of known infection types, and the other 20 were negative control samples (Table 1).
[0053] Table 1 The type and number of samples tested
[0054]
[0055] Take 300ul of sputum from each sample into a new 1.5ml centrifuge tube, add 300ul of sputum digestion liquid WT, vortex and mix, incubate at 37°C for 30min, vortex every 5-10min during digestion, and finally make the sputum sample homogeneous qualitative.
[0056] 2. RNA extraction
[0057] A common commercial RNA extraction kit (Tiangen or Qiagen) was used to extract nucleic acid from each sample.
[0058] (...
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