A composition for improving the stability of phage preparations and its application
A composition and phage technology, applied in the field of biotechnology and microbial products, can solve the problems of low survival rate, poor phage storage effect, and short phage storage time, and achieve the effects of cost reduction, good and stable protection, and high activity
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Embodiment 1
[0038] Example 1 Screening of Components of Compositions of the Invention
[0039] Select 12 kinds of common protective agent added components to carry out single factor experiments respectively: prepare and sterilize according to the concentration in Table 1, and set up a control group without adding protective agent, a total of 13 groups; among which groups 1-12 each protective agent The percentages are all g / L, and the balance is deionized water.
[0040] Table 1 Component concentrations of 13 phage protection agents and their sterilization methods
[0041]
[0042]
[0043] The protective agent components in the above Table 1 are uniformly mixed with Escherichia coli phage in a volume ratio of 1:1 under sterile conditions to obtain an Escherichia coli phage preparation. And placed at 37 ℃ for accelerated storage test at a constant temperature, the titer was detected, and the storage effect of each component was compared.
[0044] The results are shown in Table 2. U...
Embodiment 2
[0048] Example 2 Screening of the optimal component addition amount of the composition of the present invention
[0049] Among the above-mentioned protective agent components, the three kinds of magnesium sulfate, gelatin and potassium behenate with the best effect were screened for the working addition amount.
[0050] Magnesium sulfate
[0051] Under sterile conditions, different amounts of magnesium sulfate were mixed with Escherichia coli phage in a volume ratio of 1:1 to obtain an Escherichia coli phage preparation. And placed at 37 ℃ for accelerated storage test at constant temperature, the titer was detected, and the storage effects of different additions were compared.
[0052] The results are shown in Table 3. Under the storage temperature of 37 °C, the titers of 15g / L and 40g / L were reduced by 1 order of magnitude after the 5th day of storage, and 5 orders of magnitude after the 30th day of storage; it can be seen from Table 3 When the addition amount is between 20...
Embodiment 3
[0065] Example 3 Screening of the best combination of the composition of the present invention
[0066] The best protective agent components selected above are combined to screen the best composition.
[0067] Composition 1
[0068] Sodium chloride 6g / L, magnesium sulfate 30g / L, gelatin 5mL / L, glycine 0.1g / L, and water are prepared into composition 1 according to the above-mentioned component additions;
[0069] Composition 2
[0070] Add sodium chloride 6g / L, magnesium sulfate 30g / L, gelatin 5mL / L, glycine 0.1g / L, sodium dihydrogen phosphate 0.1g / L, dipotassium hydrogen phosphate 0.1g / L, and water according to the above components. Formulated into composition 2;
[0071] Composition 3
[0072] Sodium chloride 6g / L, magnesium sulfate 30g / L, gelatin 5mL / L, glycine 0.1g / L, sodium dihydrogen phosphate 0.1g / L, dipotassium hydrogen phosphate 0.1g / L, glycerin 20mL / L, water according to The above-mentioned component addition amount is formulated into composition 3
[0073] Comp...
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