Method for reducing pesticide residues and jointly killing insects by utilizing beauveria bassiana

A technology for pesticide residues and Beauveria bassiana, applied in the fields of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the three "R" problems of chemical pesticides that have not been solved and cannot achieve fungal biological control It can not completely suppress the pest population and other problems, so as to avoid the rampant regeneration of pests, excellent insecticidal activity, and reduce the cost of pest control.

Inactive Publication Date: 2021-08-06
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this mixed use method still has many problems: first, the concentration of chemical pesticides can not completely suppress the high-density pest population; second, the three "R" problems caused by chemical pesticides have not been solved; Incompatible with fungal insecticides such as B. angiospermis, it will cause the death of the fungus, and in fact, it will not achieve the biological control effect of the fungus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of operation step of utilizing Beauveria bassiana to reduce pesticide residues and combined insecticide is as follows:

[0036] (1) Activation of strains

[0037] Beauveria bassiana ( Beauveria bassiana ) were inoculated on the SDAY slant medium, and cultured at 20°C for 8 days to obtain slant strains.

[0038] The formula of SDAY medium is: 40g glucose, 10g peptone, 10g yeast extract powder and 15g agar, add water to 1000mL.

[0039] (2) Expansion of training

[0040] The slant bacteria were inoculated into SDY liquid medium, and each slant bacteria was inoculated into 300mL liquid medium, and cultured in a conical flask at 20°C and 110rpm / min shaker flask for 3 days, then, according to 1% volume weight Ratio (V / W) was inoculated into wheat solid medium for cultivation. The wheat is pre-soaked in water at a weight ratio of 1:0.2 for 0.5 hour and then sterilized by conventional high pressure.

[0041] SDY liquid medium, namely glucose 40g, yeast extract powd...

Embodiment 2

[0048] A kind of operation step of utilizing Beauveria bassiana to reduce pesticide residues and combined insecticide is as follows:

[0049] 2.1 Strain activation

[0050] Beauveria bassiana ( Beauveria amorpha ) were inoculated into SDAY slant medium and cultured at 35°C for 16 days.

[0051]The formula of SDAY medium is: 40g glucose, 10g peptone, 10g yeast extract powder and 15g agar, add water to 1000mL.

[0052] 2.2 Expansion of cultivation

[0053] The above-mentioned activated Beauveria bassiana slant strains were inoculated into SDY liquid medium, and each slant strain was inoculated into 1000mL liquid medium, and cultured in a conical flask at 30°C and 250rpm / min shake flask for 5 days, then, According to 10% volume to weight ratio (V / W), it was inoculated into rice solid culture medium for cultivation. The rice is pre-soaked in water at a weight ratio of 1:1.0 for 12 hours and then sterilized by conventional high pressure.

[0054] 2.3 Collecting spores

[005...

Embodiment 3

[0060] A kind of operation step of utilizing Beauveria bassiana to reduce pesticide residues and combined insecticide is as follows:

[0061] 3.1 Strain activation

[0062] Beauveria bassiana ( Beauveria brongniartii ) were inoculated into PDA slant medium and cultured at 30°C for 12 days.

[0063] The formula of PDA medium is: 200g peeled potato, 20g glucose and 15g agar, add water to 1000mL.

[0064] 3.2 Expansion of cultivation

[0065] The above-mentioned activated Beauveria bassiana slant strains were inoculated into PDB liquid medium, each slant strain was inoculated into 600mL liquid medium, and cultured in a conical flask at 25°C and 180rpm / min shake flask for 4 days, then, Inoculate into SDY liquid medium according to 1% volume ratio (V / V), culture at 20°C for 5 days in a shaker with a rotation speed of 110rpm / min, and obtain a liquid culture, that is, a biological agent that reduces pesticide residues and kills insects .

[0066] The formula of PDB medium is: 2...

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Abstract

The invention relates to a method for reducing pesticide residues and jointly killing pests by utilizing beauveria bassiana, and belongs to the field of bioremediation and pest control. According to the method, chemical pesticide is applied to quickly reduce population density, and then beauveria bassiana with the effect of degrading residual pesticide is applied to decompose the pesticide, so that pesticide residues are reduced, and meanwhile, the beauveria bassiana can further kill pesticide-resistant pests and prevent the pests from outbreak again. The beauveria bassiana is fungus of genus Beauveria such as Beauveria bassiana, Beauveria brongniartii, Beauveria amorpha or Beauveria velata. According to the operation technology, the advantages of chemical pesticides and biological pesticides are utilized, the pests can be rapidly controlled, the three-R problem of the chemical pesticides is avoided, the biological pesticides can continuously control the pests, and therefore the dosage of the chemical pesticides is reduced, the pest control cost is reduced, and the agricultural and forestry product quality is improved.

Description

technical field [0001] The invention belongs to the field of bioremediation and pest control, and in particular relates to a method for using Beauveria bassiana to reduce chemical pesticide residues and simultaneously exert joint control of pest population density. Background technique [0002] At present, chemical pesticides are still the main pesticides used by countries all over the world, but due to the three "R" problems of chemical pesticides (pesticide residues, pest resistance, and rampant pest regeneration), countries all over the world are trying to use biological pesticides. Among them, fungal Biopesticides mainly invade from the surface of pests, and control many types of pests. At present, the application is showing a rapid growth trend. However, the speed of fungal insecticide killing is not satisfactory, especially when the population density of fungi It can make the pests die gradually, but in the process of the pests' death, the damage to the crops is still ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01N25/32A01P7/04C12N1/14C12R1/645
CPCA01N25/32A01N63/30C12N1/14
Inventor 胡丰林季桂英陆瑞利董曲亨程仁杰
Owner ANHUI AGRICULTURAL UNIVERSITY
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