Preparation method and application of NK cell synergistic stimulation polymer micelle

A technology of co-stimulation and NK cells, applied in the field of biomedicine, can solve problems such as short biological half-life and capillary leakage, and achieve the effects of promoting apoptosis, increasing particle size and reducing glomerular filtration rate.

Active Publication Date: 2021-08-06
NORTHWESTERN POLYTECHNICAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the short biological half-life of free cytokines such as IL-2 in clinical applications, high-dose injection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of NK cell synergistic stimulation polymer micelle
  • Preparation method and application of NK cell synergistic stimulation polymer micelle
  • Preparation method and application of NK cell synergistic stimulation polymer micelle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Add 260 μl 200,000 U / ml IL-2 and 520 μl 200,000 U / ml IL-15 to the aqueous solution, then add TCEP (0.018 μmol, Beijing Yinuokai Technology Co., Ltd.), and stir the reaction at room temperature for 30- 40min;

[0062] Then add 0.6 mg of DBM-PEG-PTMC to the solution, and continue to stir and react at room temperature for 1 hour; Change the dialysis water every 4 hours);

[0063] After the dialysis, the sample was collected, centrifuged at 2000rpm / min (1000-2000rpm / min) for 10min (5-20min), the flocculent precipitate in the lower layer was discarded, the supernatant was collected, and concentrated by ultrafiltration with a 30KD ultrafiltration tube to obtain PEG-PTMC- IL-2 / IL-15 co-stimulatory polymer micelles.

[0064] The specific process parameters can be adjusted within the above range.

Embodiment 2

[0065] Embodiment 2: Inventive synthetic mechanism sees figure 1

[0066] This embodiment is different from Example 1 in that the DBM-PEG-PTMC used is synthesized by the following method:

[0067] 1) Diblock copolymer N 3 -Synthesis of PEG-PTMC: N 3 -PEG-OH (0.18mmol, 900mg, TCI (Shanghai) Chemical Industry Development Co., Ltd.), TMC (6.3mmol, 643.14mg, TCI (Shanghai) Chemical Industry Development Co., Ltd.) was dissolved in 5mL DMF, and then Add catalyst TBD (0.09mmol, 12.51mg, TCI (Shanghai) Chemical Industry Development Co., Ltd.) to the solution to initiate polymerization, and stir the reaction at room temperature for about 24h; after the reaction is completed, the reaction solution is rotated to remove some DMF, then the mixture was precipitated twice in glacial ether, and the precipitate was dried in a vacuum oven at room temperature to obtain a white solid product N 3 -PEG-PTMC;

[0068] 2) Synthesis of alkynyl-DBM: anhydrous potassium carbonate (1.79g, 12.9mmol),...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method and application of NK cell synergistic stimulation polymer micelle. According to the preparation method of the NK cell synergistic stimulation polymer micelle, IL-15 and IL-2 are simultaneously loaded by using PEG-PTMC polymer to prepare the NK cell synergistic stimulation polymeric micelle (PEG-PTMC-IL-2/IL-15). The prepared NK cell synergistic stimulation polymeric micelle has high tumor inhibition rate, and is capable of effectively promoting apoptosis of tumor cells.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and application of NK cell co-stimulatory polymer micelles. Background technique [0002] Cancer is one of the major diseases that seriously affect human health and threaten life in today's society. Natural killer cells (NK cells) are one of the main anti-tumor immune cells in the body. They can directly kill tumors and virus-infected cells without prior sensitization, and have received extensive attention in recent years. However, the killing function of NK cells on tumor cells is limited by various factors. How to efficiently activate NK cells in the process of immunotherapy is one of the problems to be solved urgently. [0003] Co-stimulation of multiple activating receptors on the surface of NK cells is a necessary prerequisite for the full activation of NK cells. Interleukin-15 (Interleukin-15, IL-15) is a soluble cytokine secreted by i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/107A61K38/20A61K47/34A61P35/00C08G81/00
CPCA61K38/2086A61K38/2013A61K9/1075A61K47/34C08G81/00A61P35/00A61K2300/00
Inventor 邵东燕黄庆生朱博博白婷孔杰师俊玲
Owner NORTHWESTERN POLYTECHNICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap