Liquid culture method for rapidly increasing biomass of didymodon tectorum protonemata

Abstract

The invention discloses a liquid culture method for rapidly increasing the biomass of didymodon tectorum protonemata. The liquid culture method comprises the following steps: taking didymodon tectorum spores, carrying out plant tissue rapid propagation culture on a solid Knop culture medium, carrying outsterile culture ofdidymodon tectorum on a solid culture medium forsubculture at a temperature of 24 DEG C, under a condition of 24 hours of illumination and 24 hours of dark, culturing in an incubator with the light intensity of 3000 lx for 1 week, taking the material, mechanically grinding and crushing, inoculating the material into an improved liquid Knop culture bottle by a pipette, culturing in the incubator with the temperature of 24 DEG C, the illumination of 12 hours, the darkness of 12 hours and the light intensity of 3000 lx for one month, observing the growth state of protonemata by using a microscope during the period, and measuring and counting the biomass of protonemata. According to the invention, a large amount of didymodon tectorum protonemata can be obtained in a short time, and the application potential of didymodon tectorum protonemata as a transgenic material subsequently is greatly improved.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a liquid culture method for rapidly increasing the biomass of P. Background technique [0002] Liverwort is one of the oldest terrestrial plants. Its evolutionary position is behind algae and before ferns and seed plants. It belongs to the sister clade of single-origin system with vascular plants, and has an extremely important research status. There are about 20,000 species in the world, all over every corner of the earth except the ocean, and even grow on deserts, tundra and rocks. They are drought-resistant, cold-resistant, and highly adaptable. They are the pioneers and pioneers of nature. [0003] Didymodon tectorum is a plant of the genus Didymodon in the family Pottiaceae. The plant body is relatively thick, yellow-green or slightly yellow-brown, densely clustered, 1-1.5cm high. This species belongs to the East Asia-North America distribution species, the distributio...

AI Technical Summary

Problems solved by technology

However, as the main source of transgenic materials, the growth state and quantity of the protonema are the main factors limiting the transgenic efficiency. The reproductive efficiency of the existing technology is low, and there is an urgent need for improved methods to increase the reproductive efficiency.

Images