VX2 rabbit liver transplantation tumor model

A transplanted tumor and model technology, applied in the field of biomedicine, can solve the problems of poor single tumor formation, low tumor formation rate, and many complications, and achieve low probability of abdominal implantation, metastasis and abdominal infection, and high tumor formation rate Effect

Inactive Publication Date: 2021-08-31
ZHONGSHAN HOSPITAL FUDAN UNIV
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: the rabbit VX2 liver tumor model in the prior art has many complications, low tu

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0012] In order to make the present invention more comprehensible, preferred embodiments are described in detail as follows.

[0013] A VX2 rabbit liver xenograft tumor model, the construction method comprising the steps of:

[0014] Step (1): Tumor formation in rabbits bearing VX2 tumor mass

[0015] First of all, young rabbits (New Zealand white rabbits with a size of about 1000g) were selected as tumor-bearing rabbits. The immune system of young rabbits is not yet perfect, and muscle tumors are easy to form in young rabbits. Rabbit anesthesia drug preparation, pentobarbital sodium powder preparation concentration 3% (3g / 100ml NS), anesthesia drug dosage is 1ml / kg, after about 10 minutes of intramuscular injection; advantages: first, simple operation, only one person is required It can be completed quickly and does not need to be fixed; secondly, it is more important than intravenous injection that the rabbit will not die because the drug enters the blood too quickly; final...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a VX2 rabbit liver transplantation tumor model. A construction method of the VX2 rabbit liver transplantation tumor model comprises the steps as follows: firstly, an anesthetic for intramuscular injection is prepared, the concentration of the anesthetic is 3% pentobarbital sodium, and the dosage is 1 mL/kg; then the VX2 tumor block for intramuscular injection is processed into a tumor block with the size of 1*1*1 mm<3>, the tumor block is inoculated into the body of a young rabbit in the anesthetic state for tumor formation, and the tumor formation parts are rabbit thigh muscle parts on the two sides of the young rabbit; and finally, separated VX2 tumor tissue is rapidly stored in PBS (Phosphate Buffer Solution) at 4 DEG C, muscular tissues around the tumor are removed by using a cell operation table, the tumor block is cut into 1*1*1 mm<3>, and the tumor block is transplanted into the liver of a healthy adult rabbit for planting. The VX2 rabbit liver transplantation tumor model is high in tumor formation rate, a formed tumor is a single tumor generally, the probability of abdominal cavity implantation metastasis and abdominal cavity infection is low, and follow-up experimental research is facilitated.

Description

technical field [0001] The invention relates to a VX2 rabbit liver transplantation tumor model, which belongs to the technical field of biomedicine. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma, HCC) is one of the most common malignant tumors. Its global incidence rate ranks fifth among men, its cancer-related mortality rate ranks second, and its incidence among women ranks seventh. Ranked sixth. About 85% of them occur in developing countries, especially in areas where hepatitis B virus (hepatic B virus, HBV) is prevalent (Southeast Asia and Africa), where the incidence of liver cancer in China accounts for 46.6% of the global liver cancer incidence. There are usually no obvious symptoms in the early stage of liver cancer. Except for a small number of early liver cancer patients who can receive radical treatment, the vast majority of liver cancer patients are in the middle and late stage when they are discovered, and have lost the opport...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2227/107A01K2267/03
Inventor 杨敏捷杜楠张雯罗剑钧马婧嵚张子寒周馨颜志平
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap