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CuS nanoparticle protein hydrogel and preparation method and application thereof

A nanoparticle and protein water technology, applied in the fields of biomedicine and polymer materials, can solve the problems of secondary wounds, affecting later activities, and limiting the preparation and application of self-healing hydrogels

Pending Publication Date: 2021-08-31
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary wound dressings, such as gauze, need to be replaced frequently, which often leads to secondary wounds, prolongs healing time, and affects later activities
Although injectable self-healing hydrogel wound dressings can solve the above problems well, the synthesis process of self-healing hydrogels is complicated, which limits the preparation and application of self-healing hydrogels

Method used

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  • CuS nanoparticle protein hydrogel and preparation method and application thereof
  • CuS nanoparticle protein hydrogel and preparation method and application thereof
  • CuS nanoparticle protein hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of PBS-EDTA-2Na solution

[0026] Mix 600mg of phosphate buffer solution powder PBS and 93mg of ethylenediaminetetraacetic acid disodium EDTA-2Na powder and dissolve in 50mL of deionized water. After constant volume, adjust the pH value to 8.0 to configure PBS-EDTA-2Na solution .

[0027] (2) Synthesis of mercaptogelatin

[0028] Dissolve 1g of gelatin in 40mL of the PBS-EDTA-2Na solution obtained in step (1), in a hot water bath at 50°C until completely dissolved, then add 32mg of Traut's Reagent, and stir magnetically for 2-3 hours to make it After fully reacting, 10 mL of deionized water was added for dialysis for 5 to 7 days, and then freeze-dried to obtain a mercaptogelatin product.

[0029] (3) Preparation of aqueous solution of CuS nanoparticles

[0030] 17.8mg of CuCl 2 2H 2 O and 22.8 mg of sodium citrate were dissolved in 20 mL of deionized water and stirred magnetically for 5 minutes at room temperature. Then 24.0 mg of Na was added to t...

Embodiment 2

[0035] (1) Preparation of PBS-EDTA-2Na solution

[0036] Mix 600mg of phosphate buffer solution powder PBS and 93mg of ethylenediaminetetraacetic acid disodium EDTA-2Na powder and dissolve in 50mL of deionized water. After constant volume, adjust the pH value to 8.0 to configure PBS-EDTA-2Na solution .

[0037] (2) Synthesis of mercaptogelatin

[0038] Dissolve 1g of gelatin in 40mL of the PBS-EDTA-2Na solution obtained in step (1), in a hot water bath at 50°C until completely dissolved, then add 32mg of Traut's Reagent, and stir magnetically for 2-3 hours to make it After fully reacting, 10 mL of deionized water was added for dialysis for 5 to 7 days, and then freeze-dried to obtain a mercaptogelatin product.

[0039] (3) Preparation of aqueous solution of CuS nanoparticles

[0040] 17.8mg of CuCl 2 2H 2 O and 22.8 mg of sodium citrate were dissolved in 20 mL of deionized water and stirred magnetically for 5 minutes at room temperature. Then 24.0 mg of Na was added to t...

Embodiment 3

[0048] (1) Preparation of PBS-EDTA-2Na solution

[0049] Mix 600mg of phosphate buffer solution powder PBS and 93mg of ethylenediaminetetraacetic acid disodium EDTA-2Na powder and dissolve in 50mL of deionized water. After constant volume, adjust the pH value to 8.0 to configure PBS-EDTA-2Na solution .

[0050] (2) Synthesis of mercaptogelatin

[0051] Dissolve 1g of gelatin in 40mL of the PBS-EDTA-2Na solution obtained in step (1), in a hot water bath at 50°C until completely dissolved, then add 32mg of Traut's Reagent, and stir magnetically for 2-3 hours to make it After fully reacting, 10 mL of deionized water was added for dialysis for 5 to 7 days, and then freeze-dried to obtain a mercaptogelatin product.

[0052] (3) Preparation of aqueous solution of CuS nanoparticles

[0053] 17.8mg of CuCl 2 2H 2 O and 22.8 mg of sodium citrate were dissolved in 20 mL of deionized water and stirred magnetically for 5 minutes at room temperature. Then 24.0 mg of Na was added to t...

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Abstract

The invention provides CuS nanoparticle protein hydrogel and a preparation method and application thereof, and belongs to the technical field of biomedicine and polymer materials. According to the invention, an antibacterial protein hydrogel with excellent self-healing performance and injectable performance is prepared through coordination of mercapto gelatin disulfide bonds and metal ions. The antibacterial protein hydrogel has a good antibacterial property through NIR laser irradiation, so that the antibacterial protein hydrogel has a wide application prospect in the biomedical fields of wound dressings, implant coatings, anti-biofilms, tissue engineering, etc.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and polymer materials, and in particular relates to a CuS nanoparticle protein hydrogel and its preparation method and application. Background technique [0002] When the skin is traumatized, it is difficult to heal itself. Long-term exposure of skin wounds is prone to produce bacteria, which affects wound healing. Skin wound dressings are necessary for wound healing. Ordinary wound dressings, such as gauze, need to be replaced frequently, which often leads to secondary wounds, prolongs the healing time, and affects later activities. Although injectable self-healing hydrogel wound dressings can solve the above problems well, the synthesis process of self-healing hydrogels is complicated, which limits the preparation and application of self-healing hydrogels. Contents of the invention [0003] Aiming at the deficiencies in the prior art, the present invention provides a CuS nanoparticle prot...

Claims

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Application Information

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IPC IPC(8): A61L27/02A61L27/22A61L27/30A61L27/34A61L27/50A61L27/52A61L27/54A61L27/58A61L26/00C08J3/075C08L89/00
CPCA61L27/34A61L27/306A61L27/222A61L27/025A61L27/54A61L27/52A61L27/58A61L27/50A61L26/0038A61L26/0004A61L26/0061A61L26/0066A61L26/008A61L26/009C08J3/075C08J2389/00A61L2300/102A61L2300/404A61L2400/06A61L2400/12A61L2430/12C08L89/00
Inventor 王淼尹纬玲潘国庆王云龙
Owner JIANGSU UNIV
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