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Injectable self-healing drug-loaded protein hydrogel as well as preparation method and application thereof

A protein water and self-healing technology, applied in the field of biomedical polymer materials, can solve the problems of sudden release and loss of KGN, complex hydrogel preparation process, and increased risk of postoperative infection, so as to reduce infection risk, excellent self-healing and injectable performance, and the effect of simple clinical operation

Active Publication Date: 2021-09-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Articular cartilage repair has always been a major problem in clinical medicine. Current studies have shown that Kartogenin (KGN, molecular formula C 20 h 15 NO 3 ) can effectively promote the differentiation of bone marrow mesenchymal stem cells into chondrocytes, thereby achieving the effect of cartilage repair, but the preparation process of the studied hydrogel is complicated and time-consuming, and KGN is very easy to be released and lost suddenly in the human body. The content of KGN loaded in most hydrogels is low, and multiple injections are required in clinical operations, which greatly increases the risk of postoperative infection

Method used

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  • Injectable self-healing drug-loaded protein hydrogel as well as preparation method and application thereof
  • Injectable self-healing drug-loaded protein hydrogel as well as preparation method and application thereof
  • Injectable self-healing drug-loaded protein hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of PBS-EDTA-2Na solution:

[0025] Mix 600mg of phosphate buffer solution powder PBS with 93mg of ethylenediaminetetraacetic acid disodium (EDTA-2Na) powder and dissolve in 50mL of deionized water, adjust the pH value to 8.0 after constant volume to obtain PBS-EDTA-2Na solution .

[0026] (2) Synthesis of mercapto gelatin:

[0027] Dissolve 1g of gelatin in 40mL of the PBS-EDTA-2Na solution obtained in step (1), put it in a hot water bath at 50°C until it is completely dissolved, then add 32mg of Traut's Reagent, and stir it magnetically for 2 hours to make it fully react , followed by adding 10 mL of deionized water for dialysis for 5 days, and freeze-drying to obtain a mercaptogelatin product.

[0028] (3) Preparation of injectable self-healing drug-loaded protein hydrogel:

[0029] Dissolve 10 mg of mono(6-mercapto-6-deoxy)beta-cyclodextrin (S-β-CD) in 70 μL of PBS to prepare S-β-CD solution, and dissolve 3 mg of KGN in 10 μL of DMSO to prepare KGN...

Embodiment 2

[0031] (1) Preparation of PBS-EDTA-2Na solution:

[0032] Mix 590mg of phosphate buffered saline solution powder (PBS) and 90mg of ethylenediaminetetraacetic acid disodium (EDTA-2Na) powder in 40mL of deionized water and dissolve in 40mL of deionized water, adjust the pH value to 8.0 after constant volume to obtain PBS-EDTA- 2Na solution.

[0033] (2) Synthesis of mercapto gelatin:

[0034] Dissolve 990mg of gelatin in 35mL of the PBS-EDTA-2Na solution obtained in step (1), in a hot water bath at 50°C until completely dissolved, then add 30mg of Traut's Reagent, stir for 3 hours with magnetic force to fully react , followed by adding 10 mL of deionized water for dialysis for 7 days, and freeze-drying to obtain a mercaptogelatin product.

[0035] (3) Preparation of injectable self-healing drug-loaded protein hydrogel:

[0036] Dissolve 9 mg of mono(6-mercapto-6-deoxy)beta-cyclodextrin (S-β-CD) in 80 μL of PBS to prepare S-β-CD solution, and dissolve 1 mg of KGN in 9 μL of DM...

Embodiment 3

[0039] (1) Preparation of PBS-EDTA-2Na solution

[0040] Mix 610mg of phosphate buffered saline solution powder (PBS) with 100mg of ethylenediaminetetraacetic acid disodium (EDTA-2Na) powder and dissolve in 50mL of deionized water, adjust the pH value to 8.0 after constant volume, thus configuring PBS- EDTA-2Na solution.

[0041] (2) Synthesis of mercaptogelatin

[0042] Dissolve 1100mg of gelatin in 50mL of the PBS-EDTA-2Na solution obtained in step (1), in a hot water bath at 50°C until completely dissolved, then add 25mg of Traut's Reagent, and stir magnetically for 2-3 hours to make it After fully reacting, 10 mL of deionized water was added for dialysis for 5 to 7 days, and then freeze-dried to obtain a mercaptogelatin product.

[0043] (3) Preparation of injectable self-healing drug-loaded protein hydrogel

[0044] Dissolve 11 mg of mono(6-mercapto-6-deoxy)beta-cyclodextrin (S-β-CD) in 70 μL of PBS to prepare S-β-CD solution, and dissolve 3 mg of KGN in 10 μL of DMSO to...

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Abstract

The invention provides injectable self-healing drug-loaded protein hydrogel as well as a preparation method and application thereof, and belongs to the field of biomedical high polymer materials. According to the injectable self-healing drug-loaded protein hydrogel and the preparation method thereof, a crosslinking point based on a ligand-metal coordination bond is formed through the coordination binding effect of a disulfide bond contained in sulfydryl gelatin and Au < + >, and then the injectable self-healing drug-loaded protein hydrogel loaded with the KGN drug is prepared; the injectable self-healing drug-loaded protein hydrogel has good mechanical properties and excellent self-healing and injectable properties, and has good application in cartilage repair.

Description

technical field [0001] The invention belongs to the field of biomedical polymer materials, in particular to an injectable self-healing drug-loaded protein hydrogel and its preparation method and application. Background technique [0002] There are many pathogenic factors for articular cartilage defects. Articular cartilage injuries caused by trauma, osteoarthritis, and osteochondrosis are very common in clinical practice, and have a high incidence and prevalence in the population. Due to the lack of blood vessels and lymphatic distribution, once the articular cartilage is damaged, its ability to repair itself is very limited. Articular cartilage repair has always been a major problem in clinical medicine. Current studies have shown that Kartogenin (KGN, molecular formula C 20 h 15 NO 3 ) can effectively promote the differentiation of bone marrow mesenchymal stem cells into chondrocytes, thereby achieving the effect of cartilage repair, but the preparation process of the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/04A61L27/20A61L27/50A61L27/52A61L27/54A61L27/56C08H1/00
CPCA61L27/222A61L27/047A61L27/20A61L27/50A61L27/52A61L27/54A61L27/56C08H1/00A61L2430/06A61L2400/06A61L2300/602C08L5/16
Inventor 王淼尹维玲王云龙潘国庆
Owner JIANGSU UNIV
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