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Force spectrum analysis method and analysis device based on nanopore sequencing

An analysis device and nanopore technology, which are applied in the field of force spectrometry analysis methods and analysis devices, can solve the problem of not having the ability to finely describe the microscopic features of the internal structure of nanoporins, and achieve the effect of high sensitivity

Active Publication Date: 2021-08-31
INST OF PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

However, the current application of nanopore sequencers lacks the means to accurately calculate the interaction between DNA sequences and nanopore proteins, and does not have the ability to further refine the microscopic characteristics of the internal structure of nanopore proteins

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  • Force spectrum analysis method and analysis device based on nanopore sequencing
  • Force spectrum analysis method and analysis device based on nanopore sequencing
  • Force spectrum analysis method and analysis device based on nanopore sequencing

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Embodiment Construction

[0033] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0034] The invention provides a differential force spectrum analysis method, which aims to obtain the force spectrum characteristics of the interaction between a single base and a protein molecule by analyzing the relevant information of nanopore sequencing, so as to quantitatively analyze the relevant microscopic physical mechanism.

[0035] figure 1 A schematic diagram showing the principle of nanopore sequencing. During a nanopore sequencing operation, DNA double strands bind and unwind with nanopore proteins embedded in biomembranes under the action of molecular motors such as helicase, and then move across the nanopore due to the action of a voltage bias. The nanopore has a sequencing-sensitive site for recording the ion c...

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Abstract

The invention provides a differential force spectrum analysis device for nanopores, which comprises: a solution tank with electrolyte solution containing biomolecules having a known base sequence; a dielectric film used for accommodating nanopores to be analyzed and dividing the solution tank into two chambers; an electric field generator used for generating an electric field applied to the solution tank; an electric signal detector used for detecting generated current data when the biomolecules pass through the nanopores under the drive of the electric field; and a control module used for obtaining the residence time of each basic group of the biomolecules passing through the nanopore according to the current data, so as to determine the differential force distribution of the interaction between the nanopores and the biomolecules, which can represent at least one characteristic of the nanopores. According to the method and the device, which are provided by the invention, the interaction between the single basic group and a protein can be quantitatively calculated with high precision, and the internal microstructure characteristics of the protein can be further accurately determined.

Description

technical field [0001] The invention relates to the field of biomolecules, in particular to a force spectrum analysis method and an analysis device for obtaining protein internal structures based on nanopore sequencing technology. Background technique [0002] Nanopore refers to a small hole with a diameter of nanometer scale on a two-dimensional film. The DNA sequencing technology based on nanopore is becoming more and more mature. The nanopore sequencing technology uses an insulating dielectric membrane to isolate the electrolyte solution (ionic liquid), which is divided into two electrolyte solution pools, wherein there is a nanoscale pore on the insulating dielectric membrane. The nanopore is the only channel for ions and molecules. When a voltage is applied, the ions in the electrolyte solution move directionally under the force of the electric field to generate an electric current. When there are DNA molecules in the electrolyte solution, the DNA molecules are also d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/36C12M1/34C12Q1/6869G01N27/00
CPCC12Q1/6869G01N27/00C12Q2535/122C12Q2565/631C12Q2537/165C12Q2565/607
Inventor 赵新佳陈晓钰米壮单欣岩陆兴华
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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