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Force spectroscopy analysis method and analysis device based on nanopore sequencing

An analysis device and nanopore technology, which is applied in the field of force spectroscopy analysis methods and analysis devices, can solve the problem of not having the ability to describe the microscopic characteristics of the internal structure of nanoporin in a fine-grained manner, and achieve the effect of high sensitivity

Active Publication Date: 2022-07-15
INST OF PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

However, the current application of nanopore sequencers lacks the means to accurately calculate the interaction between DNA sequences and nanopore proteins, and does not have the ability to further refine the microscopic characteristics of the internal structure of nanopore proteins

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  • Force spectroscopy analysis method and analysis device based on nanopore sequencing
  • Force spectroscopy analysis method and analysis device based on nanopore sequencing
  • Force spectroscopy analysis method and analysis device based on nanopore sequencing

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Embodiment Construction

[0033] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings through specific embodiments.

[0034] The invention provides a differential force spectrum analysis method, which aims to obtain the force spectrum characteristics of the interaction between a single base and a protein molecule by analyzing the related information of nanopore sequencing, so that the related microphysical mechanism can be quantitatively analyzed.

[0035] figure 1 A schematic diagram showing the principle of nanopore sequencing is shown. During a nanopore sequencing operation, the DNA duplex binds to and uncoils with nanoporins embedded in biological membranes under the action of molecular motors such as helicases, and then moves across the nanopore due to a voltage bias. The nanopore has a sequencing-sensitive site for recording the ion c...

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Abstract

The present invention provides a differential force spectroscopy analysis device for nanopores, comprising: a solution pool with an electrolyte solution containing biomolecules with a known base sequence; a dielectric membrane for accommodating a to-be-analyzed The nanopore separates the solution cell into two chambers; an electric field generator, which generates an electric field applied to the solution cell; and a control module for obtaining the residence time of each base of the biomolecule passing through the nanopore according to the current data, thereby determining the interaction between the nanopore and the biomolecule that can characterize at least one characteristic of the nanopore differential force distribution. The method and device of the present invention can quantitatively calculate the interaction between a single base and a protein with high precision, and further accurately determine the internal microstructural features of the protein.

Description

technical field [0001] The invention relates to the field of biomolecules, in particular to a force spectrum analysis method and an analysis device for obtaining the internal structure of a protein based on a nanopore sequencing technology. Background technique [0002] Nanopores refer to small pores with nanometer-scale diameters on two-dimensional films, and DNA sequencing technology based on nanopores is becoming more and more mature. The nanopore sequencing technology isolates the electrolyte solution (ionic liquid) with an insulating dielectric film, which is divided into two electrolyte solution pools, and there is a nano-scale small hole on the insulating dielectric film. The nanopore is the only channel for ions and molecules. When a voltage is applied, the ions in the electrolyte solution are moved in a direction by the electric force to generate an electric current. When DNA molecules are present in the electrolyte solution, the DNA molecules are also driven by t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42C12M1/36C12M1/34C12Q1/6869G01N27/00
CPCC12Q1/6869G01N27/00C12Q2535/122C12Q2565/631C12Q2537/165C12Q2565/607
Inventor 赵新佳陈晓钰米壮单欣岩陆兴华
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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