Bacterium and MOF-based carrier hybrid material as well as preparation method and application thereof

A hybrid material, MOF technology, applied in the field of bacterial and MOF-based carrier hybrid materials and its preparation, can solve the problems of single treatment mode and poor precision of traditional nano-therapeutic agents, so as to enhance the curative effect of tumor treatment and improve the efficiency of tumor suppression , Reduce the effect of off-target toxic side effects

Active Publication Date: 2021-09-07
WUHAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, traditional nanotherapeutics have the defects of poor precision and single treatment mode. Therefore, how to develop a kind of nanomaterial with high precision and multiple anti-tumor treatment modes has become an urgent technical problem to be solved.

Method used

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  • Bacterium and MOF-based carrier hybrid material as well as preparation method and application thereof
  • Bacterium and MOF-based carrier hybrid material as well as preparation method and application thereof
  • Bacterium and MOF-based carrier hybrid material as well as preparation method and application thereof

Examples

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preparation example Construction

[0045] According to another typical embodiment of the present invention, a method for preparing a bacteria-MOF-based carrier hybrid material is provided, such as Figure 6 As shown, the method includes:

[0046] S1. Loading MOF nanoparticles with therapeutic agents to obtain MOF carriers loaded with chemotherapeutic drugs;

[0047] As an optional implementation, the MOF nanoparticles are synthesized using a rate-controlled titration method, specifically including:

[0048] Synthesis of MIL-101-NH by Controlled Rate Titration 2 (Fe)MOF, specifically as follows: 10-50mM metal precursor and 10-50mM ligand precursor were added to the reactor containing 8-16mL DMF / water mixed solvent at 10-30mL / h through separate pipelines from the syringe pump The reaction was carried out under stirring at 50°C. After the reaction is complete, take out the reaction solution, centrifuge (10000-12000rpm, 10-20min), and wash to obtain MIL-101-NH with regular shape and size. 2 (Fe)MOF, ie said MOF...

Embodiment 1

[0089] Example 1 Shewanella bacteria combined with iron-based MOF hybrid active material and preparation method thereof

[0090] (1) Configure metal precursor solution (40mM ferric chloride aqueous solution) and ligand precursor solution (2-amino-terephthalic acid DMF / water (V / V=1 / 1) solution of 40mM), then mix the above precursor The solution was fed into a reactor containing 10 mL of DMF / water (1 / 3) mixed solvent at 20 mL / h through an independent pipeline by a syringe pump, and reacted at 50° C. for 24 h while stirring. Subsequently, centrifugation (11000rpm, 15min) and washing to obtain MIL-101-NH with regular shape and size 2 (Fe)MOFs.

[0091] (2) 10 mg of MIL-101-NH obtained in the above (1) 2 The (Fe)MOF was uniformly dispersed in 10 mL of aqueous solution, and was fully dispersed by ultrasonication for 10 min. Subsequently, 1 mL of DOX (0.5 mg / mL) aqueous solution was added to the above-mentioned nano-MOF dispersion, and stirred at room temperature in the dark for 2...

Embodiment 2

[0096] Example 2 Shewanella bacteria combined with iron-based MOF hybrid active material and preparation method thereof

[0097] (1) Configure metal precursor solution (10mM ferric chloride aqueous solution) and ligand precursor solution (2-amino-terephthalic acid DMF / water (V / V=1 / 1) solution of 10mM), then mix the above precursor The solution was fed into a reactor containing 8 mL of DMF / water (1 / 3) mixed solvent at 10 mL / h through an independent pipeline by a syringe pump, and reacted at 50° C. for 24 h while stirring. Subsequently, centrifugation (11000rpm, 15min) and washing to obtain MIL-101-NH with regular shape and size 2 (Fe)MOFs.

[0098] (2) 5 mg of MIL-101-NH obtained in the above (1) 2 The (Fe)MOF was uniformly dispersed in 10 mL of aqueous solution, and was fully dispersed by ultrasonication for 10 min. Subsequently, 1 mL of DOX (0.1 mg / mL) aqueous solution was added to the above-mentioned nano-MOF dispersion, and stirred at room temperature in the dark for 24 ...

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Abstract

The invention discloses a bacterium and MOF-based carrier hybrid material as well as a preparation method and application thereof, and the method comprises the following steps: loading a therapeutic agent on MOF nanoparticles to obtain a chemotherapeutic drug loaded MOF carrier; wrapping hyaluronic acid on the MOF carrier loaded with the chemotherapeutic drug to obtain an MOF carrier with electronegative surface; modifying the surface of shewanella with polyallylamine hydrochloride to obtain a shewanella solution with electropositive surface; and combining the MOF carrier with electronegative surface and the shewanella solution with electropositive surface through electrostatic interaction to obtain the bacterium and MOF-based carrier hybrid material. The bacterium and MOF-based carrier hybrid material is high in accuracy in anti-tumor treatment, can be used for multi-mode combined treatment, not only combines the advantages of each single therapy, but also can make up the respective defects, thereby generating an efficient and low-toxicity anti-tumor effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a hybrid material of bacteria and MOF-based carrier and its preparation method and application. Background technique [0002] As one of the deadliest diseases in the world, cancer has seriously threatened human life and health. At present, chemotherapy, radiation therapy, surgical therapy and high-intensity focused ultrasound therapy have been widely used in clinic, and have achieved great success in inhibiting tumor proliferation and prolonging patient survival. However, due to the complexity, diversity, and heterogeneity of tumors, current clinical treatment approaches can only achieve limited success. For example, in order to meet the needs of malignant proliferation of tumor cells, tumor cells often exhibit abnormal energy metabolism characteristics, mainly manifested as greatly increased glucose uptake and enhanced glycolysis, which eventually lead to a large accumulati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/46A61K47/22A61K47/36A61K47/32A61K35/74A61K31/704A61K31/337A61K41/00A61P35/00
CPCA61K47/46A61K47/22A61K47/36A61K47/32A61K35/74A61K31/704A61K31/337A61K41/0071A61P35/00A61K2300/00Y02A50/30
Inventor 陈巍海梁军龙王佳玮雒国凤张先正
Owner WUHAN UNIV
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