Protein re-folding device under electric field

A protein and refolding technology, applied in the field of protein refolding devices, can solve problems such as discontinuous operation, and achieve the effects of flexible operation, convenient continuous operation and high refolding efficiency

Inactive Publication Date: 2004-01-07
TSINGHUA UNIV
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  • Abstract
  • Description
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Problems solved by technology

Gu Zhenyu [J of Chromatography A, 2001, 918 (2): 311-318.] et al. pre-formed a urea gradient in the gel column, and let the denatured lysozyme pass through this gradient to perform renaturation. The initial denatured protein concentration was 17mg / ml is 90% activity yield, the whole folding process takes about 40 minutes, the operation is discontinuous

Method used

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  • Protein re-folding device under electric field
  • Protein re-folding device under electric field

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Embodiment Construction

[0019] like figure 1 As shown, the protein refolding device under the electric field designed by the present invention includes two electrode plates 1, 6 and four permeable membranes 2, 3, 4, 5. Four permeable membranes are arranged in parallel between two electrode plates 1 and 6 in sequence. An electrode chamber is formed between the first permeable membrane 2 and the positive electrode plate 1, a flushing chamber is formed between the second permeable membrane 3 and the first permeable membrane 2, and a flush chamber is formed between the third permeable membrane 4 and the second permeable membrane 3. In the sample chamber, a flushing chamber is formed between the fourth permeable membrane 5 and the third permeable membrane 4 , and an electrode chamber is formed between the fourth permeable membrane 5 and the negative electrode plate 6 .

[0020] The working process of the device of the present invention is as follows: the folding operation is carried out in five chambers ...

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Abstract

The present invention relates to protein refolding device under electric field, it includes two electrode plates, four permeable membranes; the four permeable membranes are successively and parallelly placed between the two electrode plates, in which the first permembrane and positive electrode plate form electrode chamber, the second permeable membrane and first permeable membrane form flushing chamber, the third permeable membrane and second permeable membrane form sample chamber, the fourth permeable membrane and third permeable membrane form flushing chamber, the fourth permeable membrane and negative electrode plate form electrode chamber. The present invention is flexible in operation, high in protein refolding efficiency etc.

Description

Technical field: [0001] The invention relates to a device for protein refolding under an electric field, which belongs to the field of biotechnology. Background technique: [0002] The development of genetic engineering technology makes the heterogeneous expression of protein a new and important way to produce medicine and special chemicals. Heterogeneously expressed proteins usually need to be folded in vitro to form the correct higher-order structure in order to obtain the expected biological activity. Escherichia coli is currently the main carrier for genetic engineering to produce recombinant proteins. Overexpression of heterologous proteins in it can produce inactive inclusion bodies, which have the advantage of obtaining high yields and avoiding proteolysis. protein. In order to obtain a refolded protein with natural activity, it is first necessary to dissolve the inactive inclusion body with a denaturant, but at the same time it also destroys the active structure of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/24C07K1/26G01N27/404G01N27/447
Inventor 刘铮黄星阎明卢滇楠丁富新袁乃驹
Owner TSINGHUA UNIV
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