Primer probe group and kit for human leukotriene receptor CysLTR2 mRNA RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection

A primer-probe, leukotriene technology, applied in DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of small detection range, problems with accuracy, low sensitivity, etc.

Pending Publication Date: 2021-09-14
ZHEDA DIXUN BIO GENE ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The ELISA method has the problems of small detection range and low se

Method used

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  • Primer probe group and kit for human leukotriene receptor CysLTR2 mRNA RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection
  • Primer probe group and kit for human leukotriene receptor CysLTR2 mRNA RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection
  • Primer probe group and kit for human leukotriene receptor CysLTR2 mRNA RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. The reagents and equipment involved are as follows:

[0037] 1.1 Reagents

[0038] 1.1.1 Whole blood total RNA kit (Hangzhou Xinjing Biological Reagent Development Co., Ltd., catalog number: 5201050)

[0039] 1.1.2 HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Cat. No.: E2050S)

[0040] 1.2 Main instruments

[0041] 1.2.1 Applied Biosystems TM 7300 fluorescent quantitative PCR instrument: ThermoFisher, USA

[0042] 1.2.2-80℃ low-temperature refrigerator: ThermoFisher, USA

[0043] 1.2.3 High-speed and low-temperature desktop centrifuge: Eppendorf, Germany

[0044] 1.2.4 Qubit 3 Fluorometer: ThermoFisher, USA

[0045] 2. Method

[0046] 2.1 Primer and probe design

[0047] According to the sequences of CysLTR2 and GAPDH, use Primer 6.0 software to design fluorescent quantitative primers and probes. After a series of effect verification, the primer pairs CysLTR2-F, CysLTR2-R, GAPDH-F, GAPDH-R and probes of CysLTR2 and GAPDH were obtained. E-Pr...

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Abstract

The invention relates to a primer probe group and a kit for human leukotriene receptor CysLTR2 mRNA RT-PCR (reverse transcription-polymerase chain reaction) detection, and belongs to the technical field of biological detection. The primer probe group comprises a primer CysLTR2-F, a primer CysLTR2-R and a probe C2-Probe, the nucleotide sequence of the primer CysLTR2-F is shown as SEQ ID NO.1, the nucleotide sequence of the primer CysLTR2-R is shown as SEQ ID NO.2, and the nucleotide sequence of the probe C2-Probe is shown as SEQ ID NO.3. The TaqMan real-time fluorescent quantitative one-step RT-PCR detection primer probe set is established for human CysLTR2, and detection means with high accuracy, wide detection range and high sensitivity is provided for detection of the protein.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a primer probe set and a kit for RT-PCR detection of human leukotriene receptor CysLTR2 mRNA. Background technique [0002] Leukotrienes are eicosaunsaturated acids with a conjugated triene structure isolated from the metabolites of arachidonic acid in leukocytes. It can be produced from arachidonic acid catalyzed by lipoxygenase (lipoxygenase). Although the content in the body is very low, it has high physiological activity and is a chemical mediator that triggers certain allergic reactions, inflammation, and cardiovascular diseases. Leukotrienes play an important role in inflammation of the upper and lower airways. Leukotrienes are more than 1000 times more potent than histamines in inducing nasal allergic reactions. Leukotrienes are significantly increased in both the immediate and delayed phases of allergen-induced nasal hypersensitivity reactions. Cysteinyl ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/11
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2600/166C12Q2600/106C12Q2531/113C12Q2561/101C12Q2545/101C12Q2521/107
Inventor 吴周杰吴善东刘奕蒋学翰杨旭凯李世旦钱磊
Owner ZHEDA DIXUN BIO GENE ENG
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