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Antibodies recognizing tau

An antibody, humanized antibody technology, applied in the direction of antibodies, medical preparations with inactive ingredients, anti-animal/human immunoglobulin, etc., can solve problems such as interference with microtubule assembly, neuronal network damage, etc.

Pending Publication Date: 2021-09-28
PROTHENA BIOSCI LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has also been reported that hyperphosphorylated tau interferes with microtubule assembly, which may promote disruption of neuronal networks

Method used

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  • Antibodies recognizing tau
  • Antibodies recognizing tau
  • Antibodies recognizing tau

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0363] Example 1. Identification of tau monoclonal antibody

[0364] Monoclonal antibodies against tau were generated as follows. Recombinant 383a.a. human tau (4RON) with an N-terminal His tag containing the P301S mutation [Immunogen A] or recombinant 383a.a. human tau (4RON) containing the P301S mutation lacking the N-terminal His tag [Immunogen B] Perform immunization. The immunogen was emulsified in RIBI adjuvant.

Embodiment 2

[0366] Example 2. Epitope Mapping of Antibody 3D6

[0367]A series of overlapping biotinylated peptides spanning the entire 383aa 4RON human tau protein was used to map the murine 3D6 antibody. Additional peptides were used to model potential post-translational modifications of the protein's C- and N-termini.

[0368] Biotinylated peptides were bound to individual wells of streptavidin-coated ELISA plates. Plates were blocked and treated with murine 3D6, followed by incubation with horseradish peroxidase-conjugated anti-mouse antibody. After thorough washing, OPD was applied to the plate and allowed to develop. Read the absorbance of the plate at 450 nm. Absorbance values ​​of wells without primary antibody were used for background subtraction and a threshold of 0.2 absorbance units was set for positive binding. Binding was mapped to sites within the MTBR region.

Embodiment 3

[0369] Example 3. Design of Humanized 3D6 Antibody

[0370] The starting or donor antibody for humanization was the mouse antibody 3D6. The heavy chain variable amino acid sequence of mature m3D6 is provided as SEQ ID NO:7. The light chain variable amino acid sequence of mature m3D6 is provided as SEQ ID NO:11. The heavy chain Kabat / Chothia complex CDR1, CDR2 and CDR3 amino acid sequences are provided as SEQ ID NOs: 8-10, respectively. Light chain Kabat CDR1, CDR2 and CDR3 amino acid sequences are provided as SEQ ID NOs: 12-14, respectively. Always use Kabat numbers.

[0371] The variable kappa (Vk) of 3D6 belongs to mouse Kabat subgroup 2, which corresponds to human Kabat subgroup 2, and the variable heavy chain (Vh) belongs to mouse Kabat subgroup 2c, which corresponds to human Kabat subgroup 2c. 1 Subgroup [Kabat E.A. et al., (1991), Sequences of Proteins of Immunological Interest, Fifth Edition NIH Publication No. 91-3242]. In Vk, the 16-residue Chothia CDR-L1 belongs...

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Abstract

The invention provides antibodies that specifically bind tau. The antibodies inhibit or delay tau-associated pathologies and associated symptomatic deterioration.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Application 62 / 758,421, filed November 9, 2018, and PCT Application PCT / US2018 / 059895, filed November 8, 2018, each of which is incorporated by reference for all purposes Incorporated in its entirety. [0003] References to Sequence Listings [0004] This application includes an Electronic Sequence Listing with filename 2019-11-08 536322WO_ST25.TXT, created on November 8, 2019 and containing 96,408 bytes, which is hereby incorporated by reference in its entirety for all purposes enter. Background technique [0005] Tau is a well-known human protein that can exist in a phosphorylated form (see, e.g., Goedert, Proc. ; Lee, Neuron 2: 1615-1624 (1989); Goedert, Neuron 3: 519-526 (1989); Andreadis, Biochemistry 31: 10626-10633 (1992). It is reported that Tau plays a role in stabilizing microtubules, especially In the central nervous system. Total Tau (t-tau, i.e. phosphorylated and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/44
CPCC07K16/18C07K2317/24C07K2317/76C07K2317/92C07K2317/94C07K16/461A61P25/28G01N33/6896C07K2317/565A61K2039/505C12N2015/8518G01N2800/52A61K47/6843C07K2317/52C07K2317/55C07K2317/622
Inventor 塔洛陈·S.·尼扎罗宾·巴伯尔菲利普·詹姆斯·多兰三世刘跃斯维特拉娜·亚历山大马克·E.·伦茨
Owner PROTHENA BIOSCI LTD
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