Rapid identification method for fingerprint spectrum of genuine radix paeoniae alba medicinal material

Abstract

The invention relates to the technical field of medicine detection, in particular to a rapid identification method for a fingerprint spectrum of a genuine radix paeoniae alba medicinal material. The method comprises the following steps: (1) preparing a test solution: taking a proper amount of the radix paeoniae alba genuine medicinal material, grinding into fine powder, and extracting by using a methanol solution to obtain the test solution; (2) preparing a mixed reference substance solution: precisely weighing three reference substances, namely paeoniflorin, albiflorin and hydroxypaeoniflorin, and adding methanol to prepare the mixed reference substance solution; (3) carrying out determination: respectively and precisely measuring the test solution prepared in the step (1) and the reference solution prepared in the step (2), injecting into a high performance liquid chromatograph, determining by adopting gradient elution, and recording a chromatogram; and processing the obtained fingerprint by using fingerprint software to obtain the fingerprint of the genuine radix paeoniae alba medicinal material. The method has the advantages of rapidness, stability, high precision, strong reproducibility and the like. The peak appearance time of reference substances paeoniflorin, albiflorin and hydroxypaeoniflorin is short, and the detection time can be greatly shortened.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a rapid identification method for fingerprints of authentic medicinal materials of Radix Paeoniae Alba. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] The current quantitative evaluation methods of traditional Chinese medicine use spectral or chromatographic methods to identify and measure one or several active ingredients, active ingredients or index ingredients, and most varieties only have general inspection items. Traditional Chinese medicine has always been the focus and difficulty of quality evaluation because of its complex components and chemical composition...

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Problems solved by technology

[0006] Prior art CN109632991A discloses a method for detecting and distinguishing Radix Paeoniae Alba and Radix Paeoniae Alba with wine using HPLC characteristic spectrum, and establishes the characteristic spectrum of Radix Paeoniae Alba decoction pieces and Radix Paeoniae Alba decoction pieces with wine , to determine whether the Radix Paeoniae Alba sample is wine-burned, the method can effectively, accurately and characteristically distinguish the difference between Radix Paeoniae Alba and Radix Paeoniae Alba in wine, but it cannot accurately investigate Radix Paeoniae Alba from different origins; the prior art CN105866296A discloses a Radix Radix Radix The fingerprint method of peony preparations is to establish the fingerprints of white peony preparations, determine 4 common characteristic peaks, and identify three components in them: hydroxypaeoniflorin, paeoniflorin, and paeoniflorin respectively; the fingerprint of the present invention There are fewer characteristic peaks in the spectrum, and the peak shape is not good, and individual peaks are tailed; in the research on the fingerprints of Radix Paeoniae Alba from different origins in the prior art, the HPLC and TLC fingerprints of Radix Paeoniae Alba were established, and TLC The chromatographic conditions are to absorb the reference substance solution of paeoniflorin and the solution of the test product, respectively point on the same silica gel G thin-layer plate, and use chloroform-ethyl acetate-methanol-formic acid as the developing agent to develop. This method compares Anhui and Zhejiang The Paeoniae Alba Radix from different origins has great differences in different origins, and there are also differences between different batches in the same origin. It is necessary to further compare the Radix Paeoniae Alba from more origins and batches to establish a more accurate and objective fingerprint of Paeoniae Alba

[0007] In the existing fingerprint detection method for Radix Paeoniae Alba, although the identification of the origin of Radix Paeoniae Alba or the evaluation of the quality of Radix Paeoniae Alba can be realized, the fingerprints of Radix Paeoniae Alba in the prior art Most of the spectrum detection methods take a long time to detect, and the detection is time-consuming and labor-intensive

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