Liquid chromatography detection method for 15 mycotoxins

A mycotoxin and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection efficiency and heavy workload, reduce workload, ensure accuracy and stability, and have good processing effect Effect

Pending Publication Date: 2021-11-05
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For this reason, the technical problem to be solved by the present invention is to provide a liquid quality detection method for 15 kinds of mycotoxins, which can be applied to 15 kinds of mycotoxins in common foods such as rice, flour, corn, soybean products, cakes, edible oil, meat products and fruits. Simultaneous detection of multiple mycotoxins to solve the problems of heavy workload and low detection efficiency when detecting multiple mycotoxins in different foods in the prior art

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  • Liquid chromatography detection method for 15 mycotoxins
  • Liquid chromatography detection method for 15 mycotoxins
  • Liquid chromatography detection method for 15 mycotoxins

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Embodiment Construction

[0046] 1. Instruments and equipment

[0047] (1) 2mL HPLC injection vial with blue PTFE cap;

[0048] (2) cyclone agitator, pH meter;

[0049] (3) Pipette: Eppenda 1-5mL, Eppenda 200-1000μL, Eppenda 20-200μL, Eppenda 2-20μL;

[0050] (4) 4&20ml brown vials with lids;

[0051] (5)Waters T3 chromatographic column, specification: 4.6x150mm, 3μm;

[0052] (6) Finomi guard column core, Gemini C18 4x3.0mm.

[0053] 2. Reagents

[0054] 2.1 Standards of mycotoxins

[0055] Aflatoxin (Afla) mixed standard: 0.5μg / mL aflatoxin B1, B2, G1, G2;

[0056] Fumonisin (F) mixed standard: 50μg / mL fumonisin B1, B2;

[0057] T2 toxin: 100 μg / mL;

[0058] HT-2 toxin: 100μg / mL;

[0059] Ochratoxin A (OTA): 10 μg / mL;

[0060] Cyclopiazonic acid (CPA): 100 μg / mL;

[0061] Citrinin (CIT): 100μg / mL;

[0062] Vomiting toxin (DON): 100μg / mL;

[0063] Patulin (PAT): 100 μg / mL;

[0064] Moniliformin (MON): 100 μg / mL;

[0065] Zearalenone (ZEN): 100 μg / mL.

[0066] 2.2 Isotopic internal st...

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Abstract

The invention discloses a liquid chromatography detection method for 15 mycotoxins, which comprises the following steps: step A, treating a sample: extracting the sample with an extracting solution, and filtering to obtain a to-be-detected solution; step B, preparing an isotope mixed internal standard solution: respectively preparing the isotope mixed internal standard solution by using isotope internal standard substances of 15 types of mycotoxins; step C, preparing a mixed standard substance solution: respectively preparing the mixed standard substance solution by using the standard substances of the 15 mycotoxins; step D, respectively adding isometric isotope mixed internal standard solution into the solution to be detected and the mixed standard substance solution; and step E, respectively detecting the to-be-detected solution added with the isotope mixed internal standard solution and the mixed standard substance solution in the step D by adopting an LC-MS / MS method, and calculating to obtain the content of the 15 mycotoxins in the sample. The detection method provided by the invention is rapid, sensitive, accurate and efficient, is suitable for detection of 15 mycotoxins of various matrix samples, and greatly reduces the workload of detection personnel.

Description

technical field [0001] The invention relates to the technical field of mycotoxin detection. Specifically, it is a liquid mass detection method for 15 kinds of mycotoxins. Background technique [0002] Mycotoxins are metabolites produced by fungi during the production and storage of crops, food, feed, etc., and are a class of small molecule biotoxins. There are many kinds of mycotoxins, and their toxic side effects and mechanisms are different for humans and animals. At present, the known mycotoxins that can cause toxicity to humans through food mainly include aflatoxins, fumonisins, T2 toxins, HT-2 toxins, ochratoxin A, cyclopiazonic acid, citrinin, and vomiting Toxin, patulin, moniliform toxin and zearalenone etc. Some of these mycotoxins can cause harmful effects such as carcinogenesis, liver toxicity, nephrotoxicity, neurotoxicity and induction of mutations, which seriously threaten human health. Mycotoxins can cause harm to the human body by entering the human body t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N30/04G01N2030/042
Inventor 栾玉静王芳琳黄健王瑞花孙晓宇
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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