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Collagen with triple-helix structure and preparation method thereof

A technology of triple helix and collagen, which is applied in the field of medical biomaterials, can solve the problems of application limitations, lack of biological activity of collagen, etc., and achieve the effect of overcoming the lack of triple helix structure

Pending Publication Date: 2021-11-19
尚诚怡美(成都)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, the collagen (essentially pure gelatin with a single molecular weight) obtained by bioengineering technology is limited in application because it does not have a triple helical structure and does not have the biological activity of collagen.

Method used

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  • Collagen with triple-helix structure and preparation method thereof

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Effect test

Embodiment 1-1

[0029] The embodiment of the present invention provides a mixed solution for promoting the formation of a triple helix structure. Weigh 10 g of sucrose, 1 g of potassium dihydrogen phosphate, and 1 g of calcium chloride, measure 2 ml of dimethyl sulfoxide, and 30 ml of glycerol, and add them to 100 ml Purified water, mix well.

Embodiment 2-1 3

[0030] The preparation of embodiment 2-1 triple helical collagen comprises the following steps:

[0031] (1) Preparation of collagen solution: take by weighing 20 g of fermented collagen produced by Jiangshan Juyuan Company, add 100 ml of purified water, stir to dissolve, and obtain collagen solution;

[0032] (2) Mixing: Take the solution of Example 1-1 and mix it with the above-mentioned collagen solution, and the volume ratio with the mixed solution is 10:1, and mix evenly;

[0033] (3) Cooling and heating program: rapidly cool the above mixed solution from room temperature to -20°C, keep it for 1 hour, then slowly raise the temperature to 10°C with a linear heating program, the heating time is 2 hours, and keep it at 10°C for 1 hour;

[0034] (4) Washing and drying: wash with purified water in an appropriate amount to remove sucrose, potassium dihydrogen phosphate and calcium chloride, and then spray dry to obtain triple-helical collagen.

Embodiment 2-2

[0036] The embodiment of the present invention provides a collagen with a triple helix structure and a preparation method thereof, comprising the following steps:

[0037] (1) Collagen dissolving: Weigh 17.5 g of fermented collagen produced by Jiangshan Juyuan Company, add 100 ml of purified water, stir to dissolve, and obtain a collagen solution;

[0038] (2) Mixing: Take the solution of Example 1-1 and mix it with the above-mentioned collagen solution, and the volume ratio with the mixed solution is 10:1, and mix evenly;

[0039] (3) Cooling and heating program: rapidly cool the above mixed solution from room temperature to -20°C, keep it for 1 hour, then slowly raise the temperature to 10°C with a linear heating program, the heating time is 2 hours, and keep it at 10°C for 1 hour;

[0040] (4) Washing and drying: wash with purified water in an appropriate amount to remove sucrose, potassium dihydrogen phosphate and calcium chloride, and then spray dry to obtain triple-helic...

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Abstract

The invention discloses collagen with a triple-helix structure and a preparation method thereof. The preparation method comprises the following steps: dissolving collagen prepared by using a fermentation method in purified water, adding a mixed solution of cane sugar, dimethyl sulfoxide, glycerol, monopotassium phosphate and calcium chloride, carrying out uniform mixing, rapidly cooling the mixed solution from normal temperature to -20 DEG C by adopting a temperature program within 5 min, maintaining the mixed solution at -20 DEG C for 1 hour, then slowly heating the mixed solution to 10 DEG C by adopting a linear temperature increasing program in a period of 2 hours, keeping the mixed solution at 10 DEG C for 1 hour so as to obtain a solution of the collagen with the triple-helix structure, removing cane sugar, monopotassium phosphate and calcium chloride via washing with purified water, and carrying out spray drying or freeze drying to prepare the collagen with the triple helix structure. According to the method, the problems of animal-derived virus, nucleic acid pollution, heavy metal pollution, microbial pollution and the like in the prior art are overcome. The collagen with the triple helix structure can be used as a pure and safe biological medicine material.

Description

technical field [0001] The invention belongs to the field of medical biomaterials, and in particular relates to collagen with a triple helix structure and a preparation method thereof. Background technique [0002] Collagen is the main component of the extracellular matrix and the most abundant and widely distributed protein in animals. The first characteristic of collagen is that it has a triple helix structure. [0003] Before the application of bioengineering technology, collagen was mainly extracted from animal tissues. Collagen was often extracted from calfskin, bovine Achilles tendon, and pigskin by acid dissolution, enzymatic, or acid-enzymatic methods to obtain collagen with a triple helix structure. . [0004] One of the oldest uses of collagen by humans is the gelatin industry. Traditionally, the heating method is used to destroy the triple helical structure of collagen to obtain a single-chain protein chain or peptide chain, that is, gelatin. However, gelatin ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/113
CPCC07K14/78
Inventor 高钧洪涛徐震梅
Owner 尚诚怡美(成都)生物科技有限公司
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