Antisense oligonucleotide for inhibiting tyrosinase expression and application thereof

An antisense oligonucleotide and tyrosinase technology, which is applied in the direction of cosmetic preparations, dressing preparations, skin care preparations, etc., can solve the problem of restricting the use of whitening products, the whitening effect is not obvious, and it has cytotoxicity To avoid harmful chemical substances from damaging cells, high delivery efficiency and specific targeting, and reduce melanin synthesis

Pending Publication Date: 2021-11-19
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the cumbersome extraction process, insignificant whitening effect, cytotoxicity and other unfavorable factors, the use in whitening products is limited.

Method used

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  • Antisense oligonucleotide for inhibiting tyrosinase expression and application thereof
  • Antisense oligonucleotide for inhibiting tyrosinase expression and application thereof
  • Antisense oligonucleotide for inhibiting tyrosinase expression and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] MATERIALS AND METHODS

[0038] 1.1 cells and reagents

[0039] A375 human melanoma cells were purchased by the laboratory and save.

[0040]DMEM medium, fetal bovine serum, Opti-MEM medium were purchased from GIBCO Company; Lipofectamine2000 purchased from Thermo Fisher / Thermo Fisher Scientific; Trizol was purchased from Invitrogen (USA) company; 2xSYBR Green qPCR Master Mix purchased since in Biosharp.

[0041] 1.2 primer synthesis

[0042] Human tyrosinase TYR real-time PCR primers and fluorescent quantitative GADPH primers were synthesized from Sangon Biological Engineering (Shanghai) Co., Ltd., the sequence shown in Table 1.

[0043] TABLE 1 Quantitative PCR primer

[0044] Primer name Sequence (5'-3 ') Gapdh-forward Caatgacccctcattgacc GaPDH-Reverse Gacaagcttcccgttctcag Tyr-forward TgcacagagagacgactctCTTG TYR-REVERSE Gagctgatgtatgcttgctaa

[0045] Synthetic chemically modified antisense oligonucleotides: antisense oligonucleotides sy...

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Abstract

The invention discloses antisense oligonucleotide for inhibiting tyrosinase expression and an application of the antisense oligonucleotide, and belongs to the field of biological medicine. According to the invention, antisense oligonucleotides targeting human tyrosinase (TYR) are designed by using RNA Straucture v6.2, and antisense oligonucleotides TYR-2 and TYR-5, which can effectively inhibit the expression of TYR and further reduce the generation of melanin, are screened out. The antisense oligonucleotide has relatively high delivery efficiency and specific targeting property; can efficiently target a human tyrosinase gene, so that melanin synthesis is reduced, color spots are faded, and the side effects of cell damage caused by harmful chemical substances, heavy metal deposition and the like caused by chemical whitening products are avoided. The effective data is provided for research and development of whitening cosmetics.

Description

Technical field [0001] The present invention is in the biomedical field, particularly relates to antisense oligonucleotides and their use for inhibiting the expression of tyrosinase. Background technique [0002] With the enhancement of living standards, consumer demand for whitening products are increasing, whether it is product safety or whitening effect have been highly concerned about consumers. He also developed the whitening agent put a higher demand, so select the efficient and safe ingredients to enhance the whitening effect is particularly important. [0003] Melanin is a biological pigment is melanin synthesis and secretion of cell in the body. Melanin can absorb ultraviolet radiation and dissipate 99.9% [1] , Protecting the body subjected to UV damage. Formation mechanism of melanin is: tyrosine gradually dopa, dopaquinone and dopachrome intermediate product in the oxidation catalyst tyrosinase, forming melanocytes, resulting in skin darkening [2] . In this synthetic p...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K31/7088A61K8/60A61P17/00A61Q19/02A61Q19/00
CPCC12N15/1137A61K31/7088A61K8/606A61P17/00A61Q19/02A61Q19/00C12N2310/14C12N2310/315C12N2320/30
Inventor费嘉苏睿
OwnerJINAN UNIVERSITY