Functionalized enzyme-powered nanomotors

A motor and nanotechnology, applied in the field of nanotechnology, can solve problems that hinder the use and applicability of nanomotors

Pending Publication Date: 2021-12-24
ФУНДАЦИО ИНСТИТУТ ДЕ БИОЕНДЖИНИЕРИА ДЕ КАТАЛУНИА +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This severely hinders the use and applicability of these nanomotors due to their restrictive external functionalization

Method used

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  • Functionalized enzyme-powered nanomotors
  • Functionalized enzyme-powered nanomotors
  • Functionalized enzyme-powered nanomotors

Examples

Experimental program
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example

[0106] 1. Targeting 3D bladder cancer spheroids with urease-driven nanomotors

[0107] 1.1. Method

[0108] Material

[0109] Ethanol (EtOH, 99%), methanol (MeOH, 99%), hydrochloric acid (37% in water), ammonium hydroxide (25% in water), tetraethylorthosilicate (TEOS, 99%), triethanolamine ( TEOA, 99%), cetyltrimethylammonium bromide (CTAB, 99%), 3-aminopropyltriethoxysilane (APTES, 99%), glutaraldehyde (GA, 25% in water ), urease (from Canavalia, type IX, powder, 50 000-100 000 units / g solid), urease activity assay kit (Sigma-Aldrich), urea (99.9% ), glycerin (99%), sodium borohydride powder (NaBH 4 , 98.0%), formaldehyde solution (37% in water), bovine serum albumin (lyophilized powder), 4-nitrophenol solution (10mM), sodium chloride extra pure (NaCl), anhydrous potassium chloride (KCl ), sodium dihydrogen phosphate (NaH 2 PO 4 ), Sodium Bicarbonate BioXtra (99.5-100.5%, NaHCO3), Dimethyl Sulfoxide (DMSO, 99.9%) and HS-PEG5K-NH 2 (HCl salt) was purchased from Sigma-...

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Abstract

The present invention provides an enzyme-powered nanomotor, comprising a particle with a surface, an enzyme, and a heterologous molecule; characterized in that the enzyme and the heterologous molecule are discontinuously attached over the whole surface of the particle. The invention also provides the nanomotor for use in therapy, diagnosis and prognosis, in particular, for the treatment of cancer. Additionally, the invention provides the use of the nanomotor for detecting an analyte in an isolated sample.

Description

[0001] This application claims the benefit of European Patent Application EP18382896 filed on December 5, 2018. technical field [0002] The invention belongs to the field of nanotechnology. In particular, the present invention relates to externally functionalized enzyme-driven nanomotors. The nanomotors of the present invention are particularly useful for therapy and biosensing. Background technique [0003] Catalytic microswimmers are artificial systems capable of self-propulsion due to the conversion of chemical energy into mechanical force and ultimately into active motion. [0004] Although chemically driven micro- and nanomotors have shown promising applicability in many fields, such as environmental remediation, cargo transport and delivery, tissue and cell penetration, and in vivo delivery of active drugs to the stomach, their use in biomedical Implementation in chemistries is often limited by the fuel's inherent toxicity or limited availability in living organisms...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B82B1/00
CPCB82B1/008B82Y5/00A61K47/6929A61K47/6861A61K47/60A61K38/50B82Y30/00B82Y35/00B82Y40/00C01P2004/03C01P2004/50C01P2004/64C12N9/80C12Y305/01005G01N33/582B82Y15/00
Inventor S·桑切斯·奥多涅斯T·帕蒂诺·帕迪亚尔A·C·洛普斯·霍泰劳
Owner ФУНДАЦИО ИНСТИТУТ ДЕ БИОЕНДЖИНИЕРИА ДЕ КАТАЛУНИА
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