Punicalagin purification method and application of punicalagin in preparation of alpha-glucosidase inhibitor

The technology of a punicalagin and a purification method is applied in the directions of chemical instruments and methods, esterified saccharide, sugar derivative preparation, etc., and can solve the problems of low purity of punicalagin and achieve the effects of low cost and simple process.

Active Publication Date: 2022-01-07
INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current preparation of purified punicalagin always has the disadvantage of low purity of punicalagin

Method used

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  • Punicalagin purification method and application of punicalagin in preparation of alpha-glucosidase inhibitor
  • Punicalagin purification method and application of punicalagin in preparation of alpha-glucosidase inhibitor
  • Punicalagin purification method and application of punicalagin in preparation of alpha-glucosidase inhibitor

Examples

Experimental program
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preparation example Construction

[0048] In the present invention, the preparation method of the punicalagin extract preferably includes the following steps:

[0049] The pomegranate peel powder and extractant are mixed for ultrasonic extraction to obtain punicalagin extract. The extractant is a mixed solvent of ethanol and water, and the volume percentage of ethanol in the extractant is 40-80%.

[0050] In the present invention, the particle size of the pomegranate peel powder is preferably ≤0.425 mm, more preferably 0.15-0.425 mm, most preferably 0.15-0.18 mm.

[0051] In the present invention, the preparation method of described pomegranate peel powder preferably comprises the following steps:

[0052] The pomegranate peel is dried, ground and sieved in sequence to obtain the pomegranate peel powder.

[0053] In one embodiment of the present invention, the pomegranate peel can be selected from the pomegranate peel produced in Huili, Sichuan.

[0054] In the present invention, the drying is preferably free...

Embodiment 1

[0105] Freeze-dry the Sichuan Huili pomegranate peel at a temperature of -40~-25°C, put it into a grinder, grind it at a speed of 12800rpm for 1min, and sieve it. to obtain pomegranate peel powder.

[0106] The pomegranate peel powder is according to the parameters in Table 1: the extractant is a mixed solvent of ethanol and water, the volume percentage of ethanol is 50%, the solid-to-liquid ratio is 20, the ultrasonic power is 800W, and the extraction time is 40min, and ultrasonic extraction is carried out to obtain Pomegranate An extract.

[0107] The punicalagin extract was filtered with a Buchner funnel, and after the solid components were removed, the ethanol was removed by rotary evaporation, and the water was removed by freeze-drying to obtain the punicalagin extract.

[0108] The content of punicalagin in the extract of punicalagin was determined by high performance liquid chromatography. in:

[0109] The chromatographic column is a ZORBAX SB-C18 chromatographic col...

Embodiment 2～29

[0124] The methods of Examples 2-29 are basically the same as those of Example 1, and the process parameters of the punicalagin extract are shown in Table 1.

[0125] Table 1 Part of the process parameters of Examples 1 to 29 and the content of punicalagin in the punicalagin extract

[0126]

[0127]

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Abstract

The invention relates to the technical field of punicalagin preparation, in particular to a punicalagin purification method and application of punicalagin in preparation of an alpha-glucosidase inhibitor. According to the invention, D101 macroporous resin and a C18 semi-preparative chromatographic column are adopted to carry out chromatographic column gradient elution on a punicalagin extract twice; the eluents of the chromatographic column gradient elution twice are set as methanol and water; the gradient elution procedure during each chromatographic column purification is specifically controlled; the interference of coexisting impurities is avoided; and the purity of the extracted punicalagin is improved. Results of the embodiment show that the purity of punicalagin in the punicalagin purified product obtained by the purification method provided by the invention is greater than 92%.

Description

technical field [0001] The invention relates to the technical field of punicalagin preparation, in particular to a method for purifying punicalagin and its application in the preparation of α-glucosidase inhibitors. Background technique [0002] Type 2 diabetes is a disease typically associated with excessive intake of dietary carbohydrates. In addition to restricting dietary intake, controlling carbohydrate digestion remains one of the effective strategies for the management of type 2 diabetes. Of all the drugs available, alpha-glucosidase inhibitors are the most effective at blocking carbohydrate digestion. Several commercial α-glucosidase inhibitors, such as acarbose, miglitol, and voglipose, used in the treatment of type 2 diabetes and its complications, have reported various side effects, including diarrhea, Abdominal pain and flatulence. [0003] Pomegranate peel, a major processing by-product of the pomegranate industry, is an inexpensive and abundant source of pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H13/08A61P3/10
CPCC07H1/08C07H13/08A61P3/10Y02A50/30
Inventor 甘人友刘毅刘宏艳何晓琴郭欢夏宇王楠马伟
Owner INST OF URBAN AGRI CHINESE ACADEMY OF AGRI SCI
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