Corn southern rust resistance gene close linkage marker and application thereof

A technology for southern rust and corn, which is applied in the field of closely linked markers for resistance genes to southern corn rust, can solve the problem of single source of resistance, and achieve the effects of simple operation, long-term control and low efficiency

Active Publication Date: 2022-01-07
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in the process of maize breeding in my country, most of the resistance source materials used for southern rust are from tropical inbred lines, and the resistance source is too single

Method used

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  • Corn southern rust resistance gene close linkage marker and application thereof
  • Corn southern rust resistance gene close linkage marker and application thereof
  • Corn southern rust resistance gene close linkage marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Discovery of fine mapping of resistance genes and molecular markers

[0059] 1. Fine mapping of resistance genes

[0060] Using maize inbred lines Jing 2416 and Jing 2416K as basic materials, it was configured with F 1 hybrids, the F 1 The F of Jing 2416×Jing 2416K was obtained by inbred generation 2 Populations were isolated for molecular marker analysis. All experimental materials were planted in Sanya, Hainan, where maize rust is high in the south. Sowing in mid-October, F 2 Group identification materials are randomly arranged and planted in a single row. The row length of each material was 5m, the row spacing was 0.55m, and 20-25 seedlings were reserved in each row. Corn inbred line Jing 2416K and corn inbred line Jing 2416 were set as a group of high resistance and high sensitivity control materials in every 50 rows. Field management is carried out according to general field production.

[0061] Resistance identification was carried out in Sanya, ...

Embodiment 2

[0071] Example 2, the application of molecular markers in the identification of maize southern rust resistance

[0072] Maize variety Jing 92 is an excellent inbred line selected by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences, and is the male parent of the largest maize variety Jingke 968 in my country. Jing 92 has high combining ability and excellent agronomic characters, but it is sensitive to southern rust.

[0073] In this example, the inbred line Jing 2416K, which is highly resistant to maize southern rust, was used as a source of resistance to improve the disease resistance of Jing 92. Taking Jing 2416K as the recipient parent and Jing 92 as the recurrent parent, it is planned to obtain rust-resistant Jing 92 through 3 times of backcrossing and 1 time of selfing selection. 40 backcross improved individual plants obtained by the above method were selected for screening of anti-rust Jing 92.

[0074] 1. Genotyping detection of Jing 2...

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Abstract

The invention discloses a corn southern rust resistance gene close linkage marker and an application thereof. A specific primer pair protected by the invention consists of single-stranded DNA molecules as shown in SEQ ID No. 1 and SEQ ID No. 2. A specific marker combination is designed by utilizing insertion/deletion of different material sequences, the southern rust resistance of different corn materials can be rapidly and accurately identified by utilizing the marker through a capillary electrophoresis technology, and the problems of difficult identification, long period and the like in regions except the southern rust high-incidence place can be solved; and besides, the defects of complex operation, poor stability and repeatability, high cost and the like of RFLP, RAPD, AFLP and other detection methods can be overcome. A molecular marker identification method provided by the invention is simple to operate and accurate and reliable in detection result, can be used for identification and auxiliary selective breeding of the corn southern rust disease resistance, and can solve the difficulties of long age, low efficiency and the like of conventional breeding.

Description

technical field [0001] The invention relates to a closely linked marker of maize southern rust resistance gene and its application. Background technique [0002] Corn is the grain and economic crop with the largest planting area and the highest total output in my country, and occupies an important position in the national economy. Maize disease is an important factor affecting yield and quality. With global climate change, changes in farming methods and the promotion of new varieties, the occurrence of corn diseases in my country has also changed. In the past, some secondary diseases (such as rust, tumor smut, etc.) pose a great threat. Southern corn rust is a global fungal disease caused by Puccinia polysora Underw., mainly distributed in tropical and subtropical regions, and is a very harmful airborne disease. In recent years, the disease has a tendency to spread to the north of our country. Once it breaks out and becomes popular, it will generally cause a 20-30% reducti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895A01H1/02A01H1/04
CPCC12Q1/6895A01H1/02A01H1/04C12Q2600/13C12Q2600/156
Inventor 宋伟王帅赵久然张如养苏爱国王元东邢锦丰王帅帅
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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