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Method for identifying seabuckthorn origin and/or varieties based on rapid evaporation ionization mass spectrometry

A rapid evaporation and ionization technology, applied in the field of analysis and detection, to achieve the effects of fast detection speed, stable results, high sensitivity and resolution

Active Publication Date: 2022-03-22
BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high water content of fruit samples, it is not easy to generate aerosols, so there are no reports on the application of this technology to fruit samples.

Method used

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  • Method for identifying seabuckthorn origin and/or varieties based on rapid evaporation ionization mass spectrometry
  • Method for identifying seabuckthorn origin and/or varieties based on rapid evaporation ionization mass spectrometry
  • Method for identifying seabuckthorn origin and/or varieties based on rapid evaporation ionization mass spectrometry

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Experimental program
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Embodiment 1

[0031] 1.1 Reagents and instruments

[0032] Xevo G2-XS quadrupole time-of-flight mass spectrometer, equipped with iKnife handheld sampling device (Waters, USA); Pump11 elite needle pump injection sampler (Harvard, USA); SGE 10mL syringe (TRAJAN, Australia), Milli Q ultrapure Water treatment system (Millipore, USA); Masslynx 4.1 data acquisition software, Live ID statistical analysis software (Waters, USA). Methanol, acetonitrile, isopropanol (chromatographically pure, U.S. Merck company); Leucine enkephalin (Sigma company), the experimental water is the ultrapure water prepared by Millipore ultrapure water instrument; 12 parts of seabuckthorn deep autumn red, Four parts of seabuckthorn with small fruit come from four production areas of Xinjiang, Heilongjiang, Jilin and Liaoning respectively, all of which are fresh seabuckthorn fruits.

[0033] 1.2 Mass Spectrometry Conditions

[0034] Use an iKnife electric knife to cut into the seabuckthorn sample with a cutting length of...

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Abstract

The invention discloses a method for identifying seabuckthorn origin and / or varieties based on rapid evaporation ionization mass spectrometry. The method mainly includes: using an iKnife electric knife to cut into the seabuckthorn sample to form an aerosol, introduce the aerosol into a mass spectrometer and detect it by a mass analyzer, and obtain the original mass spectrum data of seabuckthorn; perform peak matching and noise filtering on the original mass spectrum data through Live ID software , centralization, mass calibration and normalization processing to obtain the mass spectrum fingerprint data of seabuckthorn samples; then import the mass spectrum fingerprint data of all seabuckthorn samples into the data statistics Live ID software, and use principal component analysis combined with linear discriminant analysis methods to construct different Seabuckthorn statistical models of varieties and / or different production areas, and used to quickly identify the variety source and / or production area source of the sample to be tested. The invention has fast detection speed, high sensitivity and resolution, and provides a new detection means for identification of origin and species of seabuckthorn fruits.

Description

technical field [0001] The invention belongs to the field of analysis and detection. More specifically, it relates to a method for obtaining seabuckthorn mass spectrum fingerprints and identifying seabuckthorn origin and / or varieties based on rapid evaporation ionization mass spectrometry (REIMS) technology. Background technique [0002] Seabuckthorn is a plant with the same source of medicine and food. It is a perennial deciduous shrub or small tree. It is a light-loving, water-resistant, drought-tolerant, and small berry plant with excellent growth. Spherical or oval, about 5-10mm in diameter. Seabuckthorn fruit contains β-carotene, unsaturated fatty acids, amino acids, flavonoids, vitamins, trace elements and other ingredients, which can lower cholesterol in the human body and prevent atherosclerosis. At present, 19 provinces and autonomous regions including Shanxi, Shaanxi, Inner Mongolia, Hebei, Gansu, Ningxia, Liaoning, Qinghai, Sichuan, Yunnan, Guizhou, Xinjiang, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62G06K9/62
CPCG01N27/62G06F18/2132G06F18/2135
Inventor 严华张朝晖崔凤云别玮梁娜娜韩深冯鑫齐鹤鸣甄皓琮
Owner BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT
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