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A method for identifying feeding methods of Chinese mitten crabs

A technology of Chinese velvet crab and feeding method, applied in the field of aquaculture, can solve problems such as difficult identification, and achieve the effect of solving difficult identification and accurate identification results.

Active Publication Date: 2022-06-28
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The method is accurate and reliable, and can solve the problem of difficult identification of Chinese mitten crabs in feeding methods

Method used

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  • A method for identifying feeding methods of Chinese mitten crabs
  • A method for identifying feeding methods of Chinese mitten crabs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Collect samples of Eriocheir sinensis crabs with known feeding methods, and establish a control system.

[0023] From the Chinese Eriocheir crab breeding ponds, 10 to 100 adult Chinese Eriocheir crabs were collected from chilled fish, and 10 to 100 adult Chinese Eriocheir crabs were fed with compound feed. After the sample collection is completed, a control system is established, and the establishment method is as follows:

[0024] ① Take out the crab paste part and set aside;

[0025] ②Weigh 4 g of crab paste into a 250 mL stoppered flask, add 100 mg of pyrogallic acid, 2 mL of ethanol, and 4 mL of ultrapure water in sequence, and mix well. Add 10 mL of concentrated hydrochloric acid, and water bath at 70 °C for 40 min. After the flask was cooled to room temperature, extraction was carried out with a mixed solution of diethyl ether-petroleum ether in a ratio of 1:1, which was repeated twice. The solution was rotary evaporated to dryness at 40°C under reduced pre...

Embodiment 2

[0029] ①Buy 9 Chinese mitten crabs from Wuxi Sunan Aquatic Products Wholesale Market;

[0030] ② Take out the crab paste part of each Chinese velvet crab,

[0031] ③According to the establishment method of the control system, after homogenizing the crab paste, weigh 4g into a 250mL flask with a stopper, add 100mg pyrogallic acid, 2mL ethanol, and 4mL ultrapure water in sequence, and mix well. Add 10 mL of concentrated hydrochloric acid, and water bath at 70 °C for 40 min. After the flask was cooled to room temperature, extraction was carried out with a mixed solution of diethyl ether-petroleum ether in a ratio of 1:1, which was repeated twice. The solution was rotary evaporated to dryness at 40°C under reduced pressure. Add 8 mL of 2% sodium hydroxide-methanol solution, condense and reflux at 75°C for 30 min, add 7 mL of 14% boron trifluoride-methanol solution, and continue to reflux for 2 min. Rinse with a little water and cool to room temperature. Add 10 mL of n-hexane a...

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Abstract

The invention relates to a method for identifying feeding methods of Chinese mitten crabs, belonging to the technical field of aquaculture. It collects Chinese mitten crab adult crabs fed with chilled fish and Chinese mitten crabs fed with compound feed through the ponds of Chinese mitten crabs. After the sample collection is completed, a control system is established; random collection of Chinese mitten crabs being sold from the market The mitten crab, as the judgment object, obtains the data, and finally judges its feeding source by drawing and comparing with the non-metric multi-dimensional scaling method. The identification method of the feeding mode of the Chinese mitten crab provided by the invention has an accurate and reliable identification result, and can solve the problem of difficult identification of the feeding mode of the Chinese mitten crab.

Description

technical field [0001] The invention relates to a method for identifying the feeding mode of Eriocheir sinensis, belonging to the technical field of aquaculture. Background technique [0002] Chinese velvet crab is a special economic aquatic animal cultured in my country, especially in Jiangsu, Liaoning, Anhui, Hubei and other regions. During the breeding process, it is generally fed with natural food such as corn, sweet potatoes, snails, and chilled fish. In order to ensure the taste and flavor of the product, during the feeding process, fishermen prefer to feed bait based on chilled fish. However, chilled fish come from the fishing of marine or lake wild fishery resources. Long-term fishing will affect ecosystem diversity and lose some ecosystem services and functions. In order to avoid the occurrence of such ecological problems, the breeding methods of carnivorous aquatic animals such as Chinese velvet crabs are changing from traditional natural fishing bait to compound ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 宋超邹剑敏陈家长孟顺龙范立民胡庚东郑尧裘丽萍
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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