A detection kit and detection method for pathogenic nucleic acid under airtight conditions

Abstract

The invention discloses a detection kit and detection method for pathogenic nucleic acid under airtight conditions, belonging to the technical field of nucleic acid extraction, comprising a sealing box body and a sealing cover. Detergent chamber, lysis buffer chamber, second detergent chamber, elution buffer chamber, purification membrane chamber, waste liquid chamber, reaction detection chamber and openable sealing membrane provided on the channel; the detection method includes lysis buffer The chamber, the first detergent chamber, the second detergent chamber and the elution buffer chamber are isolated from the channel by a sealing membrane. After the corresponding steps are completed, the laser penetrates the sealing membrane, and centrifugally drives the liquid in the corresponding chamber to flow into the channel. The invention isolates pre-installed reagents by sealing membrane, completely non-contact laser drilling and centrifugal force drive, and chip structure design based on fluid properties, so as to realize the extraction of pathogenic nucleic acid and real-time fluorescence PCR amplification of the detection kit under the condition of airtight and complete sealing. detection.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction, in particular to a detection kit and detection method for pathogenic nucleic acid under airtight conditions. Background technique [0002] Nucleic acid analysis technology, as a powerful molecular diagnostic technology, has important applications in pathogen diagnosis, infectious disease prevention, disease monitoring and treatment, etc. Compared with conventional immunological diagnostic methods, nucleic acid analysis has outstanding advantages such as high specificity and high sensitivity. Due to the low concentration of most target genes, it is difficult to directly analyze and detect, so nucleic acid analysis methods such as PCR and LAMP based on the principle of nucleic acid amplification have become the main means of nucleic acid analysis. The complete process usually includes three steps: nucleic acid extraction, nucleic acid amplification, and nucleic acid detection. How...

AI Technical Summary

Problems solved by technology

It can be seen that the production process of magnetic beads that adsorb nucleic acid cannot achieve "no nucleic acid pollution". This nucleic acid extraction and purification method is not suitable for highly sensitive virus nucleic acid detection, and there is a risk of false positives

[0008] 4. Fluorescent PCR chamber C5 adopts constant temperature hot plate PCR reaction technology, which has low sensitivity

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